Information Package / Course Catalogue - Adnan Menderes University

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Protein Biochemistry

Course Code	: BİO525
Course Type	: Area Elective
Couse Group	: Second Cycle (Master's Degree)
Education Language	: Turkish
Work Placement	: N/A

Theory	: 2
Prt.	: 2
Credit	: 3
Lab	: 0
ECTS	: 8

Objectives of the Course

To learn the structures, functions, synthesis, degradation and analysis methods of proteins, which are the basic organic components of living things.

Course Content

Structure and classification of amino acids, peptides, structure, synthesis and metabolism of proteins and protein determination and analysis methods

Name of Lecturer(s)

Prof. Kubilay METİN

Learning Outcomes

1.	Learns and explains the structure of proteins, which are the basic components of living things.
2.	Learns and explains the functions of proteins
3.	Learns and explains the synthesis of proteins
4.	Learns and explains the metabolism of proteins
5.	Explains and applies protein determination and analysis methods.

Recommended or Required Reading

1.	Biyokimyanın Prensipleri (Lehninger), David L. Nelson & Michael M. Cox ; Nedret Kılıç, Palme Yayıncılık,2005
2.	Biyokimya I, II, Engin Gözükara, Nobel Tıp Kitabevi, İstanbul, 1997
3.	Analitical Biochemistry, David J. Holme and Hazel Peck, Prentice Hall, 1998.
4.	Protein Purification, Robert K. Scopes, Springer-Verlag, 1994

Weekly Detailed Course Contents

Week 1 - Theoretical

Amino acids, amino acids common structural features, classification of amino acids

Week 1 - Preparation Work

Qualitative amino acid determination

Week 2 - Theoretical

Features acid and base of amino acids, titration curve of amino acids

Week 2 - Preparation Work

Quantitative amino acid determination

Week 3 - Theoretical

Methods separate and identify of amino acids

Week 3 - Preparation Work

Separation of amino acids by paper chromatography

Week 4 - Theoretical

Quantify and quality analysis of amino acids

Week 4 - Preparation Work

Separation of amino acids bythin layer chromatography

Week 5 - Theoretical

Peptides, ionization behavior of peptides

Week 5 - Preparation Work

Qualitative protein determination

Week 6 - Theoretical

Biological activity of polypeptides

Week 6 - Preparation Work

Quantitative protein determination (Biüre and Lowry method)

Week 7 - Preparation Work

Quantitative protein determination (Bradford method)

Week 8 - Theoretical

Quantitative protein determination (Lowry method)

Week 9 - Theoretical

Covalent structure of proteins, three-dimensional structure of proteins, primer,

Week 9 - Preparation Work

Protein purification process

Week 10 - Theoretical

Covalent structure of proteins, three-dimensional structure of proteins, primer,

Week 10 - Preparation Work

Protein purification process

Week 11 - Theoretical

Secondary, tertiary and quaternary structure

Week 11 - Preparation Work

Homogenization and centrifuge

Week 12 - Theoretical

Denaturation of proteins,

Week 12 - Preparation Work

Dialysis

Week 13 - Theoretical

Synthesis of proteins

Week 13 - Preparation Work

Gel filtration

Week 14 - Theoretical

Protein metabolism

Week 14 - Preparation Work

SDS-PAGE and protein screening

Assessment Methods and Criteria

Type of Assessment	Count	Percent
Midterm Examination	1	%30
Final Examination	1	%70

Workload Calculation

Activities	Count	Preparation	Time	Total Work Load (hours)
Lecture - Theory	14	2	2	56
Lecture - Practice	14	2	2	56
Assignment	14	2	1	42
Individual Work	14	2	1	42
Midterm Examination	1	3	2	5
Final Examination	1	3	2	5
TOTAL WORKLOAD (hours)				206

Contribution of Learning Outcomes to Programme Outcomes

	PÇ-1	PÇ-2	PÇ-3	PÇ-4	PÇ-5	PÇ-6	PÇ-7	PÇ-8	PÇ-9	PÇ-10
OÇ-1	4	4	4	4	4	4	4	4	4	4
OÇ-2	3	3	3	3	3	3	3	3	3	3
OÇ-3	4	4	4	4	4	4	4	4	4	4
OÇ-4	3	3	3	3	3	3	3	3	3	3
OÇ-5	4	4	4	4	4	4	4	4	4	4

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