Information Package / Course Catalogue
Protein Biochemistry
Course Code: BİO525
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

The aim of this course is to teach the structure, function, synthesis, oxidation reactions of proteins and protein determination and purification.

Course Content

The structure and classification of amino acids, peptides, proteins, structure, synthesis and metabolism and protein determination and analysis methods

Name of Lecturer(s)
Prof. Kubilay METİN
Learning Outcomes
1.To be able to apprehend the Fundamental componentsof the protein structure of living things
2.To be able to comprehend the Functions of proteins
3.To be able to understand the Synthesis of proteins
4.To be able to acquire the Metabolism of proteins
5.To be able to get information about the methods of determination and analysis of proteins
Recommended or Required Reading
1.Biyokimyanın Prensipleri (Lehninger), David L. Nelson & Michael M. Cox ; Nedret Kılıç, Palme Yayıncılık,2005
2.Biyokimya I, II, Engin Gözükara, Nobel Tıp Kitabevi, İstanbul, 1997
3.Analitical Biochemistry, David J. Holme and Hazel Peck, Prentice Hall, 1998.
4.Protein Purification, Robert K. Scopes, Springer-Verlag, 1994
Weekly Detailed Course Contents
Week 1 - Theoretical
Amino acids, amino acids common structural features, classification of amino acids
Week 1 - Preparation Work
Qualitative amino acid determination
Week 2 - Theoretical
Features acid and base of amino acids, titration curve of amino acids
Week 2 - Preparation Work
Quantitative amino acid determination
Week 3 - Theoretical
Methods separate and identify of amino acids
Week 3 - Preparation Work
Separation of amino acids by paper chromatography
Week 4 - Theoretical
Quantify and quality analysis of amino acids
Week 4 - Preparation Work
Separation of amino acids bythin layer chromatography
Week 5 - Theoretical
Peptides, ionization behavior of peptides
Week 5 - Preparation Work
Qualitative protein determination
Week 6 - Theoretical
Biological activity of polypeptides
Week 6 - Preparation Work
Quantitative protein determination (Biüre and Lowry method)
Week 7 - Preparation Work
Quantitative protein determination (Bradford method)
Week 8 - Intermediate Exam
Mid exam
Week 9 - Theoretical
Covalent structure of proteins, three-dimensional structure of proteins, primer,
Week 9 - Preparation Work
Protein purification process
Week 10 - Theoretical
Covalent structure of proteins, three-dimensional structure of proteins, primer,
Week 10 - Preparation Work
Protein purification process
Week 11 - Theoretical
Secondary, tertiary and quaternary structure
Week 11 - Preparation Work
Homogenization and centrifuge
Week 12 - Theoretical
Denaturation of proteins,
Week 12 - Preparation Work
Dialysis
Week 13 - Theoretical
Synthesis of proteins
Week 13 - Preparation Work
Gel filtration
Week 14 - Theoretical
Nitrojen metabolism
Week 14 - Preparation Work
SDS-PAGE and protein screening
Week 15 - Final Exam
Final Exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory150230
Lecture - Practice150230
Assignment78056
Laboratory701070
Reading4028
Quiz4028
Midterm Examination1022
Final Examination1022
TOTAL WORKLOAD (hours)206
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
OÇ-1
4
4
4
4
4
4
4
4
4
4
OÇ-2
3
3
3
3
3
3
3
3
3
3
OÇ-3
4
4
4
4
4
4
4
4
4
4
OÇ-4
3
3
3
3
3
3
3
3
3
3
OÇ-5
4
4
4
4
4
4
4
4
4
4
Adnan Menderes University - Information Package / Course Catalogue