Information Package / Course Catalogue
Techniques in Molecular Biology I
Course Code: BİO543
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

The goal of the course is to teach general molecular biology techniques

Course Content

Genomic and plasmid DNA extraction from different organism and tissues, Nucleic acid detection and separation, mutation detection, PCR, PAGE, Agarose gel electrophoresis.

Name of Lecturer(s)
Learning Outcomes
1.To be able to comprehend biomolecule extraction from the cell
2.To be able to comprehend Primer design and PCR
3.To be able to comprehend Restriction endonucleases
4.To be able to comprehend electrophoresis techniques
5.To be able to comprehend mutation detection methods
Recommended or Required Reading
1.Lecturer notes
2.Sambrook and Russell,(2001) Molecular Clonning, Cold Spring Harbor Laboratory Press, ISBN-0-87969-577-3
3.Temizkan G.,Arda N.(Ed.)(2004),Moleküler Biyolojide Kullanılan Yöntemler,Nobel Kitapevleri,ISBN975-420-347-4
4.Levin B., (2004)Genes VIII, Pearson Education Inc. ISBN-0-19-508956-1
Weekly Detailed Course Contents
Week 1 - Theoretical
Structure of biomolecules
Week 2 - Theoretical
Concentration calculation and buffer solutions
Week 3 - Theoretical
General purification methods
Week 4 - Theoretical
DNA extraction from animals tissues
Week 5 - Theoretical
DNA extraction from plant tissues
Week 6 - Theoretical
DNA extraction from bacteria
Week 7 - Theoretical
Plazmid DNA extraction
Week 8 - Theoretical
Fungus DNA extraction
Week 9 - Theoretical
Methods working with proteins
Week 10 - Theoretical
Detection of nucleic acid concentration
Week 11 - Theoretical
Agarose gel electrophoresis
Week 12 - Intermediate Exam
Midterm Exam
Week 13 - Theoretical
PAGE (Poliacrylamide Gel Electrophoresis)
Week 14 - Theoretical
PCR (Polimerase Chain Reaction)
Week 15 - Theoretical
Restriction endonucleases
Week 16 - Theoretical
RNA extraction
Week 17 - Final Exam
Final Exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory152260
Lecture - Practice151245
Assignment210224
Midterm Examination128230
Final Examination138240
TOTAL WORKLOAD (hours)199
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
OÇ-1
4
4
4
4
4
4
4
4
4
4
OÇ-2
3
3
3
3
3
3
3
3
3
3
OÇ-3
4
4
4
4
4
4
4
4
4
4
OÇ-4
3
3
3
3
3
3
3
3
3
3
OÇ-5
4
4
4
4
4
4
4
4
4
4
Adnan Menderes University - Information Package / Course Catalogue