
| Course Code | : ZTB540 |
| Course Type | : Area Elective |
| Couse Group | : Second Cycle (Master's Degree) |
| Education Language | : Turkish |
| Work Placement | : N/A |
| Theory | : 2 |
| Prt. | : 2 |
| Credit | : 3 |
| Lab | : 0 |
| ECTS | : 6 |
The aim of this course is to provide knowledge and skills related to the sampling, preparation, and analysis of plant materials. The course specifically focuses on teaching students the analysis methods used for the identification of bioactive compounds in medicinal and aromatic plants. The course aims to enhance students' abilities in detecting, analyzing, and interpreting plant compounds, thereby developing their skills in this area.
The content of the course encompasses the collection of plant materials, sampling techniques, extraction methods, and analysis techniques. Students will be taught how to properly collect plant samples and prepare them for analysis, including extraction methods for obtaining and concentrating bioactive compounds from plants. Additionally, the course covers analysis methods such as UV-Vis spectroscopy, IR spectroscopy, HPLC (High-Performance Liquid Chromatography), and GC-MS (Gas Chromatography-Mass Spectrometry).
| 1. | Understanding the methods of sampling and preparation of plant materials. |
| 2. | Obtaining plant compounds using different extraction methods. |
| 3. | Analyzing plant compounds using chromatography techniques (GC, HPLC). |
| 4. | Determining the structure of plant compounds using mass spectrometry (GC-MS). |
| 5. | Interpreting and reporting chemical analysis results accurately. |
| 6. | Keeping up-to-date with current research and technologies in plant analysis. |
| 7. | Applying laboratory safety and proper working methods while adhering to ethical principles. |
| 1. | Prenzler, P. D., Ryan, D., & Robards, K. (Eds.) (2021). Handbook of antioxidant methodology: Approaches to activity determination. (Food chemistry, function and analysis; No. 28). Royal Society of Chemistry. https://doi.org/10.1039/9781839165337 |
| 2. | Waksmundzka-Hajnos, M., & Sherma, J. (Eds.). (2010). High performance liquid chromatography in phytochemical analysis. CRC press. |
| 3. | Xu, Z., Luke R Howard, Howard, L. R., & Howard, L. R. (2012). Analysis of antioxidant-rich phytochemicals. |
| 4. | Gopi, S., Amalraj, A., & Jude, S. (2021). High-Resolution Mass Spectroscopy for Phytochemical Analysis: State-of-the-Art Applications and Techniques. https://doi.org/10.1201/9781003153146 |
| Type of Assessment | Count | Percent |
|---|---|---|
| Midterm Examination | 1 | %30 |
| Final Examination | 1 | %70 |
| Activities | Count | Preparation | Time | Total Work Load (hours) |
|---|---|---|---|---|
| Lecture - Theory | 14 | 2 | 2 | 56 |
| Lecture - Practice | 14 | 3 | 2 | 70 |
| Midterm Examination | 1 | 8 | 1 | 9 |
| Final Examination | 1 | 14 | 1 | 15 |
| TOTAL WORKLOAD (hours) | 150 | |||
PÇ-1 | PÇ-2 | PÇ-3 | PÇ-4 | PÇ-5 | PÇ-6 | PÇ-7 | PÇ-8 | PÇ-9 | PÇ-10 | |
OÇ-1 | 3 | 4 | 3 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
OÇ-2 | 3 | 4 | 3 | 4 | 5 | 3 | 4 | 5 | 4 | 4 |
OÇ-3 | 4 | 4 | 5 | 4 | 4 | 4 | 4 | 4 | 5 | 4 |
OÇ-4 | 4 | 4 | 4 | 5 | 4 | 5 | 4 | 5 | 4 | 4 |
OÇ-5 | 5 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
OÇ-6 | 3 | 4 | 5 | 5 | 5 | 5 | 5 | 4 | 4 | 4 |
OÇ-7 | 5 | 5 | 5 | 4 | 4 | 4 | 3 | 3 | 3 | 3 |