Information Package / Course Catalogue
Phytochemical Analysis Methods in Medicinal and Aromatic Plants
Course Code: ZTB540
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 6
Objectives of the Course

The aim of this course is to provide knowledge and skills related to the sampling, preparation, and analysis of plant materials. The course specifically focuses on teaching students the analysis methods used for the identification of bioactive compounds in medicinal and aromatic plants. The course aims to enhance students' abilities in detecting, analyzing, and interpreting plant compounds, thereby developing their skills in this area.

Course Content

The content of the course encompasses the collection of plant materials, sampling techniques, extraction methods, and analysis techniques. Students will be taught how to properly collect plant samples and prepare them for analysis, including extraction methods for obtaining and concentrating bioactive compounds from plants. Additionally, the course covers analysis methods such as UV-Vis spectroscopy, IR spectroscopy, HPLC (High-Performance Liquid Chromatography), and GC-MS (Gas Chromatography-Mass Spectrometry).

Name of Lecturer(s)
Learning Outcomes
1.Understanding the methods of sampling and preparation of plant materials.
2.Obtaining plant compounds using different extraction methods.
3.Analyzing plant compounds using chromatography techniques (GC, HPLC).
4.Determining the structure of plant compounds using mass spectrometry (GC-MS).
5.Interpreting and reporting chemical analysis results accurately.
6.Keeping up-to-date with current research and technologies in plant analysis.
7.Applying laboratory safety and proper working methods while adhering to ethical principles.
Recommended or Required Reading
1.Prenzler, P. D., Ryan, D., & Robards, K. (Eds.) (2021). Handbook of antioxidant methodology: Approaches to activity determination. (Food chemistry, function and analysis; No. 28). Royal Society of Chemistry. https://doi.org/10.1039/9781839165337
2.Waksmundzka-Hajnos, M., & Sherma, J. (Eds.). (2010). High performance liquid chromatography in phytochemical analysis. CRC press.
3.Xu, Z., Luke R Howard, Howard, L. R., & Howard, L. R. (2012). Analysis of antioxidant-rich phytochemicals.
4.Gopi, S., Amalraj, A., & Jude, S. (2021). High-Resolution Mass Spectroscopy for Phytochemical Analysis: State-of-the-Art Applications and Techniques. https://doi.org/10.1201/9781003153146
Weekly Detailed Course Contents
Week 1 - Theoretical & Practice
Course Objective and Goals
Week 2 - Theoretical & Practice
Introduction to secondary metabolites in medicinal plants.
Week 3 - Theoretical & Practice
Sampling and preparation methods for plant materials.
Week 4 - Theoretical & Practice
Extraction methods (aqueous, organic, supercritical).
Week 5 - Theoretical & Practice
Definition, importance, and extraction methods of essential oils.
Week 6 - Theoretical & Practice
Fundamentals and types of chromatography.
Week 7 - Theoretical & Practice
Gas chromatography (GC)/ midterm
Week 8 - Theoretical & Practice
Fundamentals of high-performance liquid chromatography (HPLC).
Week 9 - Theoretical & Practice
Introduction to antioxidants, their importance, antioxidant analysis methods, and spectrophotometry.
Week 10 - Theoretical & Practice
Examination of DPPH, FRAP, and ABTS analysis in medicinal plants.
Week 11 - Theoretical & Practice
Analysis of phenols, flavonoids, and proline in medicinal plants.
Week 12 - Theoretical & Practice
Analysis methods for other components in medicinal plants (mucilage, starch, glucomannan).
Week 13 - Theoretical & Practice
Course review.
Week 14 - Theoretical & Practice
Presentations of term projects.
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142256
Lecture - Practice143270
Midterm Examination1819
Final Examination114115
TOTAL WORKLOAD (hours)150
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
OÇ-1
3
4
3
4
4
4
4
4
4
4
OÇ-2
3
4
3
4
5
3
4
5
4
4
OÇ-3
4
4
5
4
4
4
4
4
5
4
OÇ-4
4
4
4
5
4
5
4
5
4
4
OÇ-5
5
4
4
4
4
4
4
4
4
4
OÇ-6
3
4
5
5
5
5
5
4
4
4
OÇ-7
5
5
5
4
4
4
3
3
3
3
Adnan Menderes University - Information Package / Course Catalogue
2026