Information Package / Course Catalogue
Biochemical Techniques
Course Code: KİM554
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 3
Prt.: 0
Credit: 3
Lab: 0
ECTS: 6
Objectives of the Course

This course aims to provide the students a brief knowledge of theory and practice of biochemistry lab and research.

Course Content

Spectroscopic, chromatographic, electrophoretic techniques. Techniques used for molecular identification. Purification techniques. Examples from research papers.

Name of Lecturer(s)
Learning Outcomes
1.to be able to find out the theory of biochemical techniques.
2.to be able to acquire knowledge on the chemical essence of biochemical techniques such as chromatography, electrophoresis, of biomolecules.
3.to be able to recognize the theory of modern techniques such as genomics, proteomics and MALDI-MS.
4.to be able to acquire knowledge on the structural analysis of biomolecules
5.to be able to acquire knowledge on the separation and purification of biomolecules.
Recommended or Required Reading
1.Robyt, J.F., White, B.J. (1990) Biochemical Techniques. Waveland Press, Inc. Illinois, USA. ISBN No 0-88133-556-8
2.Unpublished lecture notes. Prof. Dr. A. Alev Karagözler.
Weekly Detailed Course Contents
Week 2 - Theoretical
Spectroscopic methods. UV-is spectrometry
Week 3 - Theoretical
nfrared spectroscopy (IR). Nuclear magnetic resonance (NMR). Mass Spectroscopy (MS)
Week 4 - Theoretical
Chromatographic techniques: Types of chromatography
Week 5 - Theoretical
Adsorption, ion-exchange, partition, paper, thin layer chromatography
Week 6 - Theoretical
Gel-permeation, Affinity, Gas-Liquid, High Performance Liquid chromatographies
Week 7 - Theoretical
Electrophoretic techniques: Types of electrophoresis
Week 8 - Theoretical
Use of radioisotopes in biochemical analysis (Midterm Examination)
Week 9 - Theoretical
Qualitative and quantitative methods for determining biological molecules
Week 10 - Theoretical
Biological preparations
Week 11 - Theoretical
Purification of proteins
Week 12 - Theoretical
Enzymology
Week 13 - Theoretical
Structural analysis of biological molecules
Week 14 - Theoretical
Structural analysis of biological molecules
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory144398
Midterm Examination138240
Final Examination160262
TOTAL WORKLOAD (hours)200
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
3
1
1
1
1
OÇ-2
2
2
1
2
1
OÇ-3
2
2
1
2
1
OÇ-4
2
2
1
2
1
OÇ-5
2
2
1
2
1
Adnan Menderes University - Information Package / Course Catalogue
2026