Information Package / Course Catalogue
Methods in Enzymatic Analysis
Course Code: KİM558
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 3
Prt.: 0
Credit: 3
Lab: 0
ECTS: 6
Objectives of the Course

This course aims to teach the graduate students how to deal with the principles and methods of enzymatic analysis.

Course Content

It presents theoretical knowledge as well as principles that are necessary for practical work.

Name of Lecturer(s)
Learning Outcomes
1.to be able to define the terminology of enzymology.
2.to be able to recognize Michaelis-Menten kinetics.
3.to be able to identify the principles of enzymatic analysis.
4.to be able to acquire knowledge on how to evaluate the results of enzymatic analysis.
5.to able to acquire knowledge about special enzymatic techniques
Recommended or Required Reading
1.Principles of Enzymatic Analysis, H. U. Bergmeyer, K. Gawehn, 1978, Werlag Chemie, ISBN 3-527-25678-4
2.Enzim Bilgisi, 1994. Understanding Enzymes’dan çeviren S. Cengiz, M. Cengiz. Bilimsel ve Teknik Yayınları Çevri Vakfı.
Weekly Detailed Course Contents
Week 1 - Theoretical
Terminology, importance and limits of enzymatic analysis.
Week 2 - Theoretical
Theoretical principles: Reaction kinetics.
Week 3 - Theoretical
Determination of Michaelis constant.
Week 4 - Theoretical
Determination of metabolites.
Week 5 - Theoretical
Determination of catalytic activity of enzymes.
Week 6 - Theoretical
NAD(P) – dependant reactions.
Week 7 - Theoretical
Principles of enzyme – immunoassays.
Week 8 - Theoretical
Reagents for enzymatic analysis.
Week 9 - Theoretical
Sample handling.
Week 10 - Intermediate Exam
Midterm
Week 11 - Theoretical
Absorption photometry.
Week 12 - Theoretical
Automation of analysis.
Week 13 - Theoretical
Enzymatic analysis with radiobiochemicals.
Week 14 - Theoretical
Evaluation of experimental results.
Week 15 - Theoretical
Discussion
Week 16 - Final Exam
Final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Assignment3%45
Midterm Examination1%20
Final Examination1%35
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory140342
Assignment50525
Midterm Examination149251
Final Examination130232
TOTAL WORKLOAD (hours)150
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
4
1
1
1
1
OÇ-2
2
2
1
2
1
OÇ-3
2
2
1
2
1
OÇ-4
2
2
1
2
1
OÇ-5
2
2
1
2
1
Adnan Menderes University - Information Package / Course Catalogue
2026