The primary objective of this course is to provide graduate students with a comprehensive understanding of the molecular principles, methodologies, and applications of recombinant DNA technology in animal science. The course covers the theoretical foundations and practical applications of fundamental molecular biology techniques, including DNA isolation, restriction enzymes, cloning vectors, polymerase chain reaction (PCR), gene cloning, DNA sequencing, gene expression analysis, genome editing technologies, and recombinant protein production. Emphasis is placed on the application of recombinant DNA technologies in animal breeding, molecular genetics, genomic selection, biotechnology, disease resistance, functional genomics, and sustainable livestock production systems. Upon successful completion of the course, students will be able to critically evaluate molecular biological techniques, select appropriate recombinant DNA methodologies for research purposes, interpret molecular data, and integrate contemporary biotechnological approaches into advanced research and innovation in animal science.

1- Fundamental principles of recombinant DNA technology and its role in molecular biology. 2- Nucleic acid isolation, purification, and quality assessment methods. 3- Restriction enzymes, DNA ligases, and strategies for recombinant DNA construction. 4- Cloning vectors, host cell systems, and gene cloning techniques. 5- Polymerase Chain Reaction (PCR), quantitative real-time PCR (qPCR), and DNA amplification methods. 6- DNA sequencing technologies, next-generation sequencing (NGS), and genome analysis. 7- Gene expression analysis, recombinant protein production, and functional genomics applications. 8- Genome editing technologies (CRISPR/Cas systems) and their applications in animal biotechnology. 9- Applications of recombinant DNA technologies in animal breeding, genomic selection, disease resistance, and molecular genetics research. 10- Biosafety, ethical considerations, regulatory frameworks, and laboratory practices in recombinant DNA technology.