Information Package / Course Catalogue
Serological Diagnosis Of Protozoan Diseases
Course Code: VPR532
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 4
Objectives of the Course

To be able to apply serological tests like IFAT and ELISA used for diagnosis of diseases caused by protozoans.

Course Content

To evaluate the serological tests like IFAT and ELISA used in many important protozoan diseases, preparation of antigens for serological tests, evaluation of results of these tests.

Name of Lecturer(s)
Learning Outcomes
1.To be able to tell about basic applications of serologic tests.
2.To be able to explain how to prepare antigens.
3.To be eble to experiment on how to perform serological tests.
4.to be able to evaluate results obtained from serologic tests.
5.To able to know about the development of serological tests.
Recommended or Required Reading
1.Crowther J. R. (2001).The ELISA guidebook. Totowa, NJ : Humana Press, 421 p.
2.Crowther J. R. (1995). ELISA : theory and practice. Totowa, NJ : Humana Press, 223 p.
3.Catty, D. (1989). Antibodies, a practical approach. IRL Press. Vol I-II.
Weekly Detailed Course Contents
Week 1 - Theoretical
Biology and regulation of immune response
Week 1 - Practice
Principles of preparation of the solutions to be used in serological tests
Week 2 - Theoretical
The structure and classification of antibodies
Week 2 - Practice
Introduction to fluorescent microscopy and its working principles
Week 3 - Theoretical
Antigen - antibody interactions
Week 3 - Practice
Preparation of antigens for the IFA test
Week 4 - Theoretical
Detection of antigen-antibody interaction
Week 4 - Practice
Performing indirect IFA test- I
Week 5 - Theoretical
Immunofluorescence method
Week 5 - Practice
Performing indirect IFA test- II
Week 6 - Theoretical
Enzyme Linked Immunosorbent assay (ELISA)
Week 6 - Practice
Preparation of antigens for ELISA, introduction to the ELISA reader and program, preparation of related solutions
Week 7 - Theoretical
Evaluation of ELISA test results, determination of cut off values
Week 7 - Practice
Performing indirect ELISA- I
Week 8 - Theoretical
(Midterm exam)- Evaluation of assignments
Week 8 - Practice
Performing an ELISA test- II
Week 9 - Theoretical
Immunodiffusion and immunoelectrophoresis
Week 9 - Practice
Performing an ELISA test- III
Week 10 - Theoretical
Sabin-Feldman Dye test
Week 10 - Practice
Preparation of SDS-PAGE gel and parasite proteins
Week 11 - Theoretical
Agglutination tests
Week 11 - Practice
Elektroforesis of proteins on SDS-PAGE gel
Week 12 - Theoretical
Analysis of proteins in SDS-PAGE
Week 12 - Practice
Western blot analysis -I
Week 13 - Theoretical
Western blot analysis
Week 13 - Practice
Western blot analysis -II
Week 14 - Theoretical
Comparison of commercial kits with home-made tests
Week 14 - Practice
Sabin-Feldman test, Use of some of the commercial kits
Week 15 - Final Exam
Final exam
Week 16 - Final Exam
Final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Assignment1%5
Quiz1%5
Midterm Examination1%20
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory141128
Lecture - Practice140228
Assignment1055
Reading140114
Quiz1011
Midterm Examination110111
Final Examination112113
TOTAL WORKLOAD (hours)100
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
OÇ-1
2
3
3
4
3
2
2
5
5
1
5
5
OÇ-2
3
2
1
1
2
3
2
5
5
OÇ-3
4
2
3
4
4
3
5
2
3
1
5
5
OÇ-4
5
3
4
5
5
4
2
3
5
4
5
5
OÇ-5
5
4
5
3
3
4
5
3
5
4
1
5
Adnan Menderes University - Information Package / Course Catalogue
2026