
| Course Code | : BYF538 |
| Course Type | : Area Elective |
| Couse Group | : Second Cycle (Master's Degree) |
| Education Language | : Turkish |
| Work Placement | : N/A |
| Theory | : 2 |
| Prt. | : 2 |
| Credit | : 3 |
| Lab | : 0 |
| ECTS | : 4 |
To acquire general information on the usage and preperation of cell cultures
Introduction to cell cultures, general properties of cell cultures, frequently used cell culture lines and their general properties, selection and sterilization of the materials used in preperation of cell cultures, general laboratory rules and preperations in cell culture laboratories, general rules in cell culture innoculation, passage of cell cultures, methods to determine cell viability, cell counting, how to perform viability tests, contamination and cytotoxicity, the kinetics of cell death in cultures, storage of frozen cultures
| Lec. Mahmut Alp KILIÇ |
| 1. | Learn about the usage fields and general properties of cell cultures |
| 2. | To copmrehend the properties and growing conditions of different cell lines used in cell culture studies |
| 3. | Learn about general methods used in laboratories in cell culture studies, learn about cell counting and cell viability tests |
| 4. | Gain knowledge on important points in the studies with cell cultures, such as cytotoxicity |
| 5. | To gain the basic practical abilities related to cell culture studies |
| 1. | Culture of Animal Cells, Edit: R. Ian Freshney, Wıley-Liss Press, 2005 |
| 2. | Molecular Cell Biology, Edit: Harvey Lodish, W.H. Freeman and Company, 2008 |
| 3. | Cell and Tıssue Culture. Doyle A and Griffiths JB (eds). John Wiley&Sons. |
| 4. | Basic Cell Culture Protocols. 3rd Ed. Helgason CH, Miller CL (eds). 2004, Humana Press. |
| Type of Assessment | Count | Percent |
|---|---|---|
| Midterm Examination | 1 | %20 |
| Final Examination | 1 | %60 |
| Practice Examination | 1 | %20 |
| Activities | Count | Preparation | Time | Total Work Load (hours) |
|---|---|---|---|---|
| Lecture - Theory | 13 | 1 | 2 | 39 |
| Lecture - Practice | 13 | 1 | 2 | 39 |
| Midterm Examination | 1 | 10 | 2 | 12 |
| Final Examination | 1 | 10 | 2 | 12 |
| TOTAL WORKLOAD (hours) | 102 | |||
PÇ-1 | PÇ-2 | PÇ-3 | PÇ-4 | PÇ-5 | PÇ-6 | PÇ-7 | PÇ-8 | PÇ-9 | PÇ-10 | PÇ-11 | PÇ-12 | PÇ-13 | PÇ-14 | PÇ-15 | PÇ-16 | PÇ-17 | PÇ-18 | |
OÇ-1 | 5 | 5 | 5 | 5 | 5 | 2 | 3 | 3 | 3 | 3 | 4 | 3 | 4 | 3 | 3 | 3 | 3 | 3 |
OÇ-2 | 5 | 5 | 5 | 5 | 5 | 2 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 4 |
OÇ-3 | 5 | 5 | 5 | 5 | 5 | 2 | 3 | 3 | 3 | 3 | 4 | 3 | 4 | 3 | 3 | 3 | 3 | 3 |
OÇ-4 | 5 | 5 | 5 | 5 | 5 | 2 | 3 | 3 | 3 | 3 | 4 | 3 | 4 | 3 | 3 | 3 | 3 | 3 |
OÇ-5 | 5 | 5 | 5 | 5 | 5 | 2 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 4 |