Information Package / Course Catalogue
Cell Culture and Techniques
Course Code: BYF538
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 4
Objectives of the Course

To acquire general information on the usage and preperation of cell cultures

Course Content

Introduction to cell cultures, general properties of cell cultures, frequently used cell culture lines and their general properties, selection and sterilization of the materials used in preperation of cell cultures, general laboratory rules and preperations in cell culture laboratories, general rules in cell culture innoculation, passage of cell cultures, methods to determine cell viability, cell counting, how to perform viability tests, contamination and cytotoxicity, the kinetics of cell death in cultures, storage of frozen cultures

Name of Lecturer(s)
Lec. Mahmut Alp KILIÇ
Learning Outcomes
1.Learn about the usage fields and general properties of cell cultures
2.To copmrehend the properties and growing conditions of different cell lines used in cell culture studies
3.Learn about general methods used in laboratories in cell culture studies, learn about cell counting and cell viability tests
4.Gain knowledge on important points in the studies with cell cultures, such as cytotoxicity
5.To gain the basic practical abilities related to cell culture studies
Recommended or Required Reading
1.Culture of Animal Cells, Edit: R. Ian Freshney, Wıley-Liss Press, 2005
2.Molecular Cell Biology, Edit: Harvey Lodish, W.H. Freeman and Company, 2008
3.Cell and Tıssue Culture. Doyle A and Griffiths JB (eds). John Wiley&Sons.
4.Basic Cell Culture Protocols. 3rd Ed. Helgason CH, Miller CL (eds). 2004, Humana Press.
Weekly Detailed Course Contents
Week 1 - Theoretical
Introduction to cell cultures, general properties of cell cultures
Week 1 - Practice
Introduction to cell cultures, general properties of cell cultures
Week 2 - Theoretical
Primary cell cultures and their general properties
Week 2 - Practice
Primary cell cultures and their general properties
Week 3 - Theoretical
Immortal cell cultures and their general properties
Week 3 - Practice
Immortal cell cultures and their general properties
Week 4 - Theoretical
Monolayer cell cultures and their general properties
Week 4 - Practice
Monolayer cell cultures and their general properties
Week 5 - Theoretical
Selection and sterilization of the materials used in preperation of cell cultures, general laboratory rules and preperations in cell culture laboratories
Week 5 - Practice
Selection and sterilization of the materials used in preperation of cell cultures, general laboratory rules and preperations in cell culture laboratories
Week 6 - Theoretical
Preperation of media, sera, tripsin and other materials to be used in cultures
Week 6 - Practice
Preperation of media, sera, tripsin and other materials to be used in cultures
Week 7 - Theoretical
General rules in cell culture innoculation, cell morphology and passage of cell cultures
Week 7 - Practice
General rules in cell culture innoculation, cell morphology and passage of cell cultures
Week 8 - Intermediate Exam
Midterm exam
Week 9 - Theoretical
Control of cell culture flasks, cell tripsination
Week 9 - Practice
Control of cell culture flasks, cell tripsination
Week 10 - Theoretical
Methods to determine cell viability, cell counting, how to perform viability tests
Week 10 - Practice
Methods to determine cell viability, cell counting, how to perform viability tests
Week 11 - Theoretical
Preperation of cell plates for two and three dimentional experiments, cell innoculation
Week 11 - Practice
Preperation of cell plates for two and three dimentional experiments, cell innoculation
Week 12 - Theoretical
Collection of sferoids in three dinemtional cultures, fixation and investigation of these materials using light and electron microscopy
Week 12 - Practice
Collection of sferoids in three dinemtional cultures, fixation and investigation of these materials using light and electron microscopy
Week 13 - Theoretical
Contamination and cytotoxicity, the kinetics of cell death in cultures
Week 13 - Practice
Contamination and cytotoxicity, the kinetics of cell death in cultures
Week 14 - Theoretical
Storage of frozen cultures
Week 14 - Practice
Storage of frozen cultures
Week 15 - Final Exam
Final Exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%20
Final Examination1%60
Practice Examination1%20
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory131239
Lecture - Practice131239
Midterm Examination110212
Final Examination110212
TOTAL WORKLOAD (hours)102
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
PÇ-15
PÇ-16
PÇ-17
PÇ-18
OÇ-1
5
5
5
5
5
2
3
3
3
3
4
3
4
3
3
3
3
3
OÇ-2
5
5
5
5
5
2
3
3
3
3
3
3
3
3
3
3
3
4
OÇ-3
5
5
5
5
5
2
3
3
3
3
4
3
4
3
3
3
3
3
OÇ-4
5
5
5
5
5
2
3
3
3
3
4
3
4
3
3
3
3
3
OÇ-5
5
5
5
5
5
2
3
3
3
3
3
3
3
3
3
3
3
4
Adnan Menderes University - Information Package / Course Catalogue
2026