Information Package / Course Catalogue
Cell Culture
Course Code: BYK520
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 5
Objectives of the Course

To learn about cell culture and its properties

Course Content

In vitro propagation conditions of eukaryotic cells, mediums used in cell cultures, media used in general purpose and their contents, media improved for suspension cultures and their contents, media for specialized cells (tumors, bone, cartilage, cloth, tissue, etc.), substances such as serum and synthetic "supplemented" added to the media, use of cell cultures. Conditions required to be in the cell culture laboratories, concepts of sterility and isolation, equipment and layout of the laboratory, certainly should be and not be in the laboratory, washing, sterilization and storage. Aseptic techniques, sterilization methods, sterile manipulation, cell count, cell viability test.

Name of Lecturer(s)
Learning Outcomes
1.To know the necessary infrastructure of cell culture laboratory
2.Learning why and how cell culture is done
3.Learning the characteristics of culture cells
4.Learning the equipments used in culture
5.To know the application areas of cell culture in medical biochemistry
Recommended or Required Reading
1.Animal Cell Culture: Essential methods
2.Basic Cell Culture Protocols
3.Basic Cell Culture:J.M.Davis
Weekly Detailed Course Contents
Week 1 - Theoretical
Introduction to cell culture
Week 2 - Theoretical
Cell culture laboratory features and requirements
Week 3 - Theoretical
Sterility
Week 3 - Practice
Sterility
Week 4 - Theoretical
Media used in cell culture
Week 5 - Practice
Preparation of medium
Week 6 - Theoretical
Primary cell culture
Week 6 - Practice
Explant cell culture
Week 7 - Practice
Subculturing and cell countin
Week 8 - Intermediate Exam
Quiz
Week 9 - Theoretical
Cell Lines
Week 9 - Practice
Cryopreservation
Week 10 - Theoretical
Karyotype analysis in cell culture
Week 10 - Practice
Karyotype analysis
Week 11 - Theoretical
Cytotoxicity analysis
Week 11 - Practice
Cytotoxicity analysis
Week 12 - Practice
Cytotoxicity analysis
Week 13 - Theoretical
Apoptosis analysis
Week 13 - Practice
Apoptosis analysis
Week 14 - Theoretical
Cancer cell culture
Week 15 - Theoretical
Prenatal cell culture
Week 16 - Final Exam
Final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory81332
Lecture - Practice61324
Assignment1123
Individual Work112466
TOTAL WORKLOAD (hours)125
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
4
5
4
4
5
OÇ-2
5
5
4
4
5
OÇ-3
4
5
5
5
4
OÇ-4
5
5
5
4
5
OÇ-5
5
5
5
5
4
Adnan Menderes University - Information Package / Course Catalogue
2026