Information Package / Course Catalogue
Biochromatography
Course Code: BYK523
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 0
Credit: 2
Lab: 0
ECTS: 3
Objectives of the Course

Learning biochromatography and its usage areas

Course Content

Basic steps in biyochromatography, gel filtration, ion exchange biyochromatography, hydrophobic proteins (interaction) chromatography, affinity chromatography, dye ligand affinity chromatography, immobilized histidine ligand affinity chromatography, immobilized metal-ion affinity chromatography, thiophilic interaction chromatography, glycobiology and biochromatography, imprinted polymers as for the stationary phase in affinity purification, industrial biochromatography, biochromatography and biomedical applications

Name of Lecturer(s)
Learning Outcomes
1.To have knowledge about biomolecules
2.Having knowledge about biochromatography
3.To learn the principles and methods of chromatographic separation of biomolecules
4.To learn different chromatographic techniques for separation of biomolecules
5.Learning protein isolation techniques
Recommended or Required Reading
1.Biochromatography: Theory and Practice:M. A. Vijayalakshmi
Weekly Detailed Course Contents
Week 1 - Theoretical
Chromatography and chromatography basics
Week 2 - Theoretical
Chromatography and chromatography basics
Week 3 - Theoretical
Biomolecules
Week 4 - Theoretical
Biomolecules
Week 5 - Theoretical
Introduction to biochromatography
Week 6 - Theoretical
Separation of biomolecules by ion exchange chromatography
Week 7 - Theoretical
Separation of biomolecules by hydrophobic interaction chromatography
Week 8 - Intermediate Exam
Quiz
Week 9 - Theoretical
Chromatographic separation of biomolecules by affinity chromatography
Week 10 - Theoretical
Immunoaffinity chromatography
Week 11 - Theoretical
Chromatographic separation of biomolecules by gel filtration chromatography
Week 12 - Theoretical
Chromatographic separation of biomolecules by immobilized metal affinity chromatography (IMAC)
Week 13 - Theoretical
Protein isolation techniques
Week 14 - Theoretical
Thin layer chromatography of proteins
Week 16 - Theoretical
Chromatographic separation of biomolecules by fast protein liquid chromatography (FPLC)
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory141356
Assignment131619
TOTAL WORKLOAD (hours)75
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
4
5
4
5
4
OÇ-2
5
4
5
4
5
OÇ-3
5
4
5
4
5
OÇ-4
5
5
5
5
4
OÇ-5
4
4
4
5
5
Adnan Menderes University - Information Package / Course Catalogue
2026