Information Package / Course Catalogue
Enzymatic Analytical Methods
Course Code: BYK524
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 3
Objectives of the Course

To have information about enzymatic analysis, preanalytical process and evaluation of post-analysis results

Course Content

The importance and limits of terminology in enzymatic analysis, theoretical principles: reaction kinetics, determination of Michaelis constant, the determination of metabolites, determination of the catalytic activity of enzymes, NAD (P) - dependent reactions, principles of enzyme - immunoassay techniques, enzymatic reagents used in the analysis, sample preparation, absorption photometry, automation of analysis, enzymatic analyses using radiobiochemicals, evaluation of the experimental results

Name of Lecturer(s)
Learning Outcomes
1.To have information about structure and properties of enzymes
2.To be able to define the differences between enzymes and normal catalytic substances
3.To have information about enzymatic metabolite determination
4.Learning the principles of enzyme immunoassay technique
5.To have knowledge about preanalytical processes and postanalytic evaluations in enzymatic analysis
Recommended or Required Reading
1.Biochemistry by lehninger
2.Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis:Robert A. Copeland
Weekly Detailed Course Contents
Week 1 - Theoretical
Structure and properties of enzymes
Week 2 - Theoretical
Structure and properties of enzymes
Week 3 - Theoretical
Terminology, importance and limits of enzymatic analysis
Week 4 - Theoretical
Theoretical principles and reaction kinetics
Week 5 - Theoretical
Determination of metabolites
Week 6 - Practice
Determination of metabolites
Week 7 - Practice
Determination of Michaelis constant
Week 8 - Intermediate Exam
Quiz
Week 9 - Theoretical
Determination of catalytic activity of enzymes
Week 9 - Practice
Determination of catalytic activity of enzymes
Week 10 - Theoretical
NAD(P) dependent reactions
Week 10 - Practice
NAD(P) dependent reactions
Week 11 - Theoretical
Principles of enzyme immunoassay technique
Week 12 - Theoretical
Reagents used in enzymatic analysis, sample preparation, absorption photometry, automation of analysis, enzymatic analysis with radiobiochemicals and evaluation of experimental results
Week 13 - Theoretical
Reagents used in enzymatic analysis, sample preparation, absorption photometry, automation of analysis, enzymatic analysis with radiobiochemicals and evaluation of experimental results
Week 14 - Practice
Reagents used in enzymatic analysis, sample preparation, absorption photometry, automation of analysis, enzymatic analysis with radiobiochemicals and evaluation of experimental results
Week 15 - Practice
Reagents used in enzymatic analysis, sample preparation, absorption photometry, automation of analysis, enzymatic analysis with radiobiochemicals and evaluation of experimental results
Week 16 - Final Exam
Final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory91336
Lecture - Practice51320
Assignment131619
TOTAL WORKLOAD (hours)75
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
5
5
5
4
4
OÇ-2
4
4
5
5
4
OÇ-3
5
5
5
5
4
OÇ-4
5
4
5
4
5
OÇ-5
5
5
5
5
5
Adnan Menderes University - Information Package / Course Catalogue
2026