Information Package / Course Catalogue
Immuno- Histochemical Tecniques
Course Code: THE521
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 6
Objectives of the Course

learning immunohistochemical techniques

Course Content

techniques of demonstrating structures of interest using essentially antibodies.

Name of Lecturer(s)
Learning Outcomes
1.Cytological preparation, learning of fixation methods
2.To learn immunohistochemical monitoring and detection methods
3.Learning of antigen Retrival techniques
4.Learning of immunohistochemistry techniques in Frozen sections
5.Properties of a good antibody, high interest against antigens and learning of high binding strength
Recommended or Required Reading
Weekly Detailed Course Contents
Week 1 - Theoretical
Introduction to histochemistry and cytochemistry
Week 2 - Theoretical
Cytological preparation, fixation
Week 3 - Theoretical
Definition of immunohistochemistry, antigens and antibodies
Week 4 - Theoretical
primary antibodies
Week 5 - Theoretical
sekonder antibodies
Week 6 - Theoretical
monoklonal antibodies
Week 7 - Intermediate Exam
midterm exam
Week 8 - Theoretical
poliklonal antibodies
Week 9 - Theoretical
direct method
Week 10 - Theoretical
indirect method
Week 11 - Theoretical
Antigen Retrival techniques
Week 12 - Theoretical
Immunohistochemical staining methods Problems encountered in immunohistochemical staining
Week 13 - Theoretical
Immunohistochemistry in Frozen Sections
Week 14 - Theoretical
Consideration in selection of immunohistochemical technique
Week 15 - Theoretical
article discussion
Week 16 - Final Exam
final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142256
Lecture - Practice142256
Individual Work100440
TOTAL WORKLOAD (hours)152
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
5
4
4
4
4
OÇ-2
3
3
3
4
3
OÇ-3
4
3
4
3
4
OÇ-4
3
3
4
4
3
OÇ-5
4
3
4
4
4
Adnan Menderes University - Information Package / Course Catalogue