Information Package / Course Catalogue
Electron-microscopic Tecniques
Course Code: THE522
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 4
Objectives of the Course

Learning of electron microscopy techniques

Course Content

It includes the techniques required to examine a biological material in the electron microscope.

Name of Lecturer(s)
Prof. Alpaslan GÖKÇİMEN
Learning Outcomes
1.Electron microscopic follow-up of tissues
2.Semi-thin and thin sections
3.Be able to prepare knives for cross section
4.To be able to make electron microscopic examination of the sections and necessary evaluations
5.interpretation of the errors encountered in electron microscopy
Recommended or Required Reading
Weekly Detailed Course Contents
Week 1 - Theoretical
Transmission electron microscopy (TEM) working principles
Week 2 - Theoretical
Scanning electron microscope (SEM) working principles
Week 3 - Theoretical
Preparation of the electron microscopy laboratory
Week 4 - Theoretical
to be considered issues in the electron laboratory P laboratory
Week 5 - Theoretical
Points to be considered during tissue removal for animal or human electron microscope, Fixation (fixation), fixative selection, detection period and chemical substances used in detection solutions, Important aspects of tissue detection
Week 6 - Theoretical
tissue washing, dehydration, transparency
Week 7 - Intermediate Exam
midterm exam
Week 8 - Theoretical
Introduction to the tissue of plastic material and embedding in plastic (blocking)
Week 9 - Theoretical
Preparation of glass blades, trimming, semi-thin sectioning (ultramicrotomy), painting and examination of semi-thin sections
Week 10 - Theoretical
Properties of grids, cleaning of heaters, film coating of heaters
Week 11 - Theoretical
Important points in taking thin sections, taking the sections into the heater, contrasting the sections
Week 12 - Theoretical
Examining and evaluating the sections and taking pictures
Week 13 - Theoretical
Fixation preparation errors and results in electron microscopy
Week 14 - Theoretical
Follow-up errors and results in electron microscopy
Week 15 - Theoretical
Evaluation errors and results in electron microscopy
Week 16 - Final Exam
final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142256
Lecture - Practice141242
TOTAL WORKLOAD (hours)98
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
4
4
4
3
3
OÇ-2
3
4
4
4
4
OÇ-3
4
3
4
4
4
OÇ-4
4
3
2
4
1
OÇ-5
4
3
3
3
3
Adnan Menderes University - Information Package / Course Catalogue