Information Package / Course Catalogue
Introduction to Cell Culture
Course Code: THE531
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 6
Objectives of the Course

Creation of primary cell lines and detailed cell culture techniques using commercial cell lines

Course Content

Primary and cell lines, the general principles for the culture, the investigation of ethical problems

Name of Lecturer(s)
Prof. Kemal ERGİN
Learning Outcomes
1.To be able to indicate laboratory and consumables used in cell culture
2.To be able to apply cell culture, passaging, freezing methods by using primary cell lines
3.To be able to distinguish the necessary cell culture methods and materials for different types of cells
4.correct use and sterilization of biosafety cabinets
5.To learn sterilization techniques
Recommended or Required Reading
Weekly Detailed Course Contents
Week 1 - Theoretical
What is Cell Culture?
Week 2 - Theoretical
What is cell culture used for?
Week 3 - Theoretical
Introduction of the Cell Culture Laboratory
Week 4 - Theoretical
Cell Culture Systems
Week 5 - Theoretical
Media contents used in cell culture
Week 6 - Theoretical
comparing the differnet Media contents used in cell culture
Week 7 - Intermediate Exam
midterm exam
Week 8 - Theoretical
Sterilization in cell culture
Week 9 - Theoretical
Properties of culture vessels used in cell culture
Week 10 - Theoretical
Cell Lines and Usage
Week 11 - Theoretical
Primary cell culture and preparation
Week 12 - Theoretical
Primary cell production and culture
Week 13 - Theoretical
Cell Freezing and Storage
Week 14 - Theoretical
Biomaterials and their use in culture
Week 15 - Theoretical
3D culture media and its use
Week 16 - Final Exam
final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142256
Lecture - Practice142256
Assignment100440
TOTAL WORKLOAD (hours)152
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
3
4
3
4
3
OÇ-2
4
3
4
3
4
OÇ-3
3
4
3
4
3
OÇ-4
4
3
4
3
4
OÇ-5
3
3
4
3
4