Information Package / Course Catalogue
Equipments Used in Laboratory and Methods
Course Code: VBY529
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 1
Prt.: 2
Credit: 2
Lab: 0
ECTS: 2
Objectives of the Course

Biochemistry laboratory equipment and methods used to introduce and about them to make applications.

Course Content

Biochemical methods, pre-treatment for biological materials, Spectrometry, AAS, flame photometry, fluorometry, RIA, ELISA, HPLC, thin layer chromatography,, GC, GC-MS, lectin affinity chromatography, Introduction of ICP-AES and ICP-MS methods and make to applications in laboratories.

Name of Lecturer(s)
Learning Outcomes
1.To be able to comprehend and apply the preparatory pre-processing for the biological materials.
2.To be able to recognize the devices used in the laboratory of Biochemistry
3.Have knowledge about chromatographic systems
4.To learn about the working principles of the devices and to learn the points to be considered in practice
5.To be able to evaluate the advantages and disadvantages of different devices and methods
Recommended or Required Reading
1.Karagül H., Altıntaş A., Fidancı U.R., Sel T.(2000) Klinik Biyokimya. Medisan Yayınevi ANKARA
2.Kaplan L.A, Pesce A.J, KAzmierczak S.C. Clinical chemistry. Mosby. U.S.A.
Weekly Detailed Course Contents
Week 1 - Theoretical
Biochemical classification of methods
Week 1 - Practice
Rules need to be aware of when using devices
Week 2 - Theoretical
Süzme, santrfüj, diyaliz uygulamaları
Week 2 - Practice
Filtration, centrifugation, dialysis applications
Week 3 - Theoretical
Spectrophotometry
Week 3 - Practice
Adsorption, and microdiffusion applications
Week 4 - Theoretical
Atomic absorption Spectrophotometry (AAS)
Week 4 - Practice
Enzymatic analysis in spectrophotometer
Week 5 - Theoretical
Flame photometry
Week 5 - Practice
Colorimetric analysis in spectrophotometer
Week 6 - Theoretical
Fluorometry
Week 6 - Practice
Video display for AAS
Week 7 - Practice
Video display for RIA
Week 8 - Theoretical
Midterm exam
Week 9 - Theoretical
ELISA
Week 9 - Practice
Hormones analysis with ELISA
Week 10 - Practice
Video display for HPLC
Week 11 - Theoretical
Thin-layer chromatography
Week 11 - Practice
Thin-layer chromatography applications
Week 12 - Theoretical
Gas chromatography (GC)
Week 12 - Practice
Evalution of chromatographic methods
Week 13 - Theoretical
Gas chromatography (GC)- mass spectrometry (GC-MS)
Week 13 - Practice
İntroduction of GC-MS
Week 14 - Theoretical
Lectin affinity chromatography
Week 14 - Practice
Lectin affinity chromatography applications
Assessment Methods and Criteria
Type of AssessmentCountPercent
Quiz1%10
Midterm Examination1%20
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory140114
Lecture - Practice140228
Quiz1001
Midterm Examination1112
Final Examination1415
TOTAL WORKLOAD (hours)50
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
PÇ-15
OÇ-1
5
5
5
5
5
OÇ-2
5
5
5
OÇ-3
4
OÇ-4
4
OÇ-5
4
Adnan Menderes University - Information Package / Course Catalogue
2026