Information Package / Course Catalogue
Essentials of Geneticmanipulation
Course Code: TIB505
Course Type: Required
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 1
Prt.: 2
Credit: 2
Lab: 0
ECTS: 4
Objectives of the Course

Course Content

Name of Lecturer(s)
Prof. Mehtap KILIÇ EREN
Learning Outcomes
1.
2.2. Learning molecular biology concepts
3.4. Learning gene manipulation and cloning techniques
4.Acquiring skills for conducting molecular biolgy techniques
5.
Recommended or Required Reading
1.Molecular Cell Biology – Harvey Lodish, Arnold Berk, Chris A. Keiser, Monty Krieger, Anthony Bretscher, Hidde Ploegh, Angelika Amon, Mathew P. Scott - W. H. Freeman; Seventh Edition edition (May 2, 2012)
2.Molecular cloning: A laboratory manual – Michael Green and Joseph Sambrook – Cold Sprıng Harbor Laboratory Press (Fourth edition) 2012
Weekly Detailed Course Contents
Week 1 - Theoretical
Introduction to genetic manipulation
Week 2 - Theoretical
Restriction endonucleases
Week 3 - Theoretical
DNA modification enzymes
Week 4 - Theoretical
Vectors
Week 5 - Theoretical
cDNA synthesis
Week 6 - Theoretical
Hybridization
Week 7 - Theoretical
Recombinant DNA libraries
Week 8 - Theoretical
Promoters and their roles in gene expression (Midterm Exam)
Week 9 - Theoretical
Resctriction endonuclease digestion of DNA
Week 10 - Theoretical
in vitro mutagenesis
Week 11 - Theoretical
in vitro mutagenesis
Week 12 - Theoretical
Subcloning practice
Week 13 - Practice
Subcloning pracitce
Week 14 - Theoretical
Presentation and Discussion
Week 15 - Final Exam
Final Exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%40
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory131126
Lecture - Practice132252
Midterm Examination1527
Final Examination1729
TOTAL WORKLOAD (hours)94
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
5
1
1
1
3
OÇ-2
5
1
1
1
3
OÇ-3
2
5
5
1
3
OÇ-4
2
5
5
2
3
OÇ-5
2
3
3
2
5
Adnan Menderes University - Information Package / Course Catalogue
2026