Information Package / Course Catalogue
Method Development and Validation in Pharmacological and Toxicological Analysis
Course Code: VFT557
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 1
Prt.: 2
Credit: 2
Lab: 0
ECTS: 4
Objectives of the Course

The purpose of this course, pharmacological and toxicological analyzes, and information about the method development and validation of this method to teach.

Course Content

Pharmacology and toxicology analysis methods, chromatographic methods, chromatographic methods, method development, method validation of chromatographic methods.

Name of Lecturer(s)
Learning Outcomes
1.Pharmacology and toxicology analysis methods
2.Chromatographic methods
3.Chromatographic methods, method development
4.Chromatographic method validation methods on the knowledge and skills.
5.To find out and use resources about the profession in the area.
Recommended or Required Reading
1.Veterinary Pharmacology and Therapeutics, 8th Edition, Jim E. Riviere (Editor), Mark G. Papich (Editor), 2009.
2.Modern Pharmacology, 6th Edition, Lippincott Williams and Wilkins, 2004 (Ed. C.R. Craig and R.E. Stitzel)
3.Basic and Clinical Pharmacology, 9th Edition, McGraw-Hill, New York, 2004 (Ed. B. Katzung)
4.Goodman and Gilman’s The Pharmacological Basis of Therapeutics 11th Edition, McGraw-Hill, 2006 (Eds. Brunton, Lazo, Parker, Buxton and Blumenthal)
Weekly Detailed Course Contents
Week 1 - Theoretical
Pharmacology and toxicology analysis methods
Week 1 - Practice
The materials used in pharmacology and toxicology analysis
Week 2 - Theoretical
Chromatographic methods
Week 2 - Practice
TLC application
Week 3 - Theoretical
GC and HPLC
Week 3 - Practice
Parts of the introduction of GK
Week 4 - Theoretical
Liquid-phase extraction methods
Week 4 - Practice
Introduction to HPLC components
Week 5 - Theoretical
Solid phase extraction methods
Week 5 - Practice
HPLC-conditioning
Week 6 - Theoretical
Linearity calculation and preparation of reference standards
Week 6 - Practice
HPLC application
Week 7 - Theoretical
Calculation of the ratio of buy-back
Week 7 - Practice
HPLC application
Week 8 - Theoretical
LOD calculations (Mid-term exam)
Week 9 - Theoretical
LOQ calculations
Week 9 - Practice
GK application
Week 10 - Theoretical
Detection range, and precision (precision) calculation
Week 10 - Practice
GK application
Week 11 - Theoretical
Linearity, Specificity / Selectivity
Week 11 - Practice
chromatogram evaluation
Week 12 - Theoretical
To determine the sensitivity and Reality
Week 12 - Practice
chromatogram evaluation
Week 13 - Theoretical
Reproducible results, the calculation
Week 13 - Practice
Liquid-liquid phase extraction
Week 14 - Theoretical
preparation of SOPs
Week 14 - Practice
The solid-liquid phase extraction
Week 15 - Final Exam
Final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Presentation1%5
Verbal Examination1%5
Midterm Examination1%20
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142142
Lecture - Practice142256
Midterm Examination1314
Final Examination1314
TOTAL WORKLOAD (hours)106
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
OÇ-1
3
3
5
OÇ-2
3
3
5
OÇ-3
3
3
5
OÇ-4
3
3
5
OÇ-5
5
5
5
Adnan Menderes University - Information Package / Course Catalogue
2026