Information Package / Course Catalogue
Methods For Biomolecules II
Course Code: MBTK521
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 10
Objectives of the Course

The goal of the course is taught general molecular biology techniques

Course Content

Several high level molecular biology techniques; gene cloning, RT-PCR, transformation, protein extraction and analysis.

Name of Lecturer(s)
Learning Outcomes
1.Understanding of recombination
2.Understanding of gene transfer
3.Understanding of gene cloning
4.Understanding of Western blotting technique
5.Understanding of RNA and RT-PCR
Recommended or Required Reading
1.Lecture notes
2.Moleküler Biyolojide Kullanılan Yöntemler (Güler Temizkan, Nazlı9 Arda)
3.Sambrook and Russell,(2001) Molecular Clonning, Cold Spring Harbor
Weekly Detailed Course Contents
Week 1 - Theoretical & Practice
Genetic recombination
Week 2 - Theoretical & Practice
Gene transfer mechanisms: Conjugation
Week 3 - Theoretical & Practice
Gene transfer mechanisms: Transformation
Week 4 - Theoretical & Practice
Detection for clones
Week 5 - Theoretical & Practice
Producing product from cloned gene (Quiz)
Week 6 - Theoretical & Practice
Multipleks PCR
Week 7 - Theoretical & Practice
Gen Polimorphisms
Week 8 - Theoretical & Practice
DNA purification from gel (Midterm exam)
Week 9 - Theoretical & Practice
RFLP
Week 10 - Theoretical & Practice
Allel spesific PCR
Week 11 - Theoretical & Practice
Nested PCR (Quiz)
Week 12 - Theoretical & Practice
Protein extraction-Protein detection methods
Week 13 - Theoretical & Practice
SDS-PAGE/ Western Blotlama
Week 14 - Theoretical & Practice
RNA extraction and RT-PCR
Assessment Methods and Criteria
Type of AssessmentCountPercent
Attending Lectures14%5
Quiz2%5
Midterm Examination1%20
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142256
Lecture - Practice143270
Presentation 54125
Reading142142
Individual Work141128
Quiz2114
Midterm Examination1819
Final Examination114115
TOTAL WORKLOAD (hours)249
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
OÇ-1
5
5
3
5
5
3
4
4
4
4
3
3
5
OÇ-2
5
5
3
5
5
3
4
4
4
4
3
3
5
OÇ-3
5
5
3
4
4
3
5
5
5
5
3
3
5
OÇ-4
5
5
3
4
4
3
5
5
5
5
3
3
5
OÇ-5
5
5
3
4
4
3
5
5
5
5
3
3
5
Adnan Menderes University - Information Package / Course Catalogue
2026