Information Package / Course Catalogue
| Course Code | : ZBY538 |
| Course Type | : Area Elective |
| Couse Group | : Second Cycle (Master's Degree) |
| Education Language | : Turkish |
| Work Placement | : N/A |
| Theory | : 2 |
| Prt. | : 0 |
| Credit | : 3 |
| Lab | : 2 |
| ECTS | : 8 |
Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The aim of this course is to learn how to obtain, identify and purify proteins from animal, plant or microbial sources.
The extraction of proteins from an animal, plant or microbial source, quantification, purification by various methods and protein visualization are performed.
| Lec. Zehra Burcu BAKIR |
| 1. | Knows the principles of homogenization |
| 2. | Knows the principles of protein precipitation |
| 3. | Knows dialysis and gel filtration |
| 4. | Knows the structure of proteins and purification methods |
| 5. | Knows PAGE and SDS-PAGE electrophoresis |
| 1. | Modern Experimental Biochemistry, Boyer R.; Benjamin Cummings, 2002 |
| 2. | Biochemical Techniques, Theory and Practice, Robyt J.F., White B.J., Waveland Press, 1990 |
| 3. | Protein purification handbook; amersaham pharmacia biotech |
| 4. | Lehninger Principles of Biochemistry, Nelson, D.L., Cox, M.M., Palme Yayıncılık, 2013 |
| 5. | Analitical Biochemistry, Holme D.J., Peck H., Prentice Hall, 1998 |
| 6. | Molecular Biology, protein synthesis and degradation, Editörler, Yıldırım A., Bardakçı F., Karataş M., Tanyolaç B., Nobel Yayın Dağıtım, 2007 |
| Type of Assessment | Count | Percent |
|---|---|---|
| Midterm Examination | 1 | %30 |
| Final Examination | 1 | %70 |
| Activities | Count | Preparation | Time | Total Work Load (hours) |
|---|---|---|---|---|
| Lecture - Theory | 14 | 4 | 2 | 84 |
| Lecture - Practice | 14 | 5 | 2 | 98 |
| Midterm Examination | 1 | 4 | 2 | 6 |
| Final Examination | 1 | 4 | 2 | 6 |
| TOTAL WORKLOAD (hours) | 194 | |||
PÇ-1 | PÇ-2 | PÇ-3 | PÇ-4 | PÇ-5 | |
OÇ-1 | 5 | 4 | 4 | 3 | 4 |
OÇ-2 | 5 | 4 | 4 | 3 | 4 |
OÇ-3 | 5 | 4 | 4 | 3 | 4 |
OÇ-4 | 5 | 5 | 5 | 5 | 5 |
OÇ-5 | 5 | 5 | 5 | 4 | 4 |