Information Package / Course Catalogue
Course Code
Course Type
Couse Group
Education Language
Work Placement
Theory: 0
Prt.: 0
Credit: 0
Lab: 0
ECTS
Objectives of the Course

Course Content

Name of Lecturer(s)
Learning Outcomes
1.Knows the principles of homogenization
2.Knows the principles of protein precipitation
3.Knows dialysis and gel filtration
4.Knows the structure of proteins and purification methods
5.Knows PAGE and SDS-PAGE electrophoresis
Recommended or Required Reading
1.Modern Experimental Biochemistry, Boyer R.; Benjamin Cummings, 2002
2.Biochemical Techniques, Theory and Practice, Robyt J.F., White B.J., Waveland Press, 1990
3.Protein purification handbook; amersaham pharmacia biotech
4.Lehninger Principles of Biochemistry, Nelson, D.L., Cox, M.M., Palme Yayıncılık, 2013
5.Analitical Biochemistry, Holme D.J., Peck H., Prentice Hall, 1998
6.Molecular Biology, protein synthesis and degradation, Editörler, Yıldırım A., Bardakçı F., Karataş M., Tanyolaç B., Nobel Yayın Dağıtım, 2007
Weekly Detailed Course Contents
Week 1 - Theoretical & Practice
Basic principles of protein purification
Week 2 - Theoretical & Practice
Homogenization and centrifugation
Week 3 - Theoretical & Practice
Precipitation of proteins and dialysis
Week 4 - Theoretical & Practice
Concentration of Protein Solutions
Week 5 - Theoretical & Practice
Column chromatography
Week 6 - Theoretical & Practice
Gel filtration chromatography
Week 7 - Theoretical & Practice
Ion exchange chromatography
Week 8 - Theoretical & Practice
Hidrophobic interaction chromatography
Week 9 - Theoretical & Practice
Affinity Chromatography and High pressure liquid chromatography
Week 10 - Theoretical & Practice
Electrophoresis
Week 11 - Theoretical & Practice
Polyacrylamide Gel Electrophoresis (PAGE)
Week 12 - Theoretical & Practice
Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
Week 13 - Theoretical & Practice
Enzyme Activity and Protein Determination
Week 14 - Theoretical & Practice
Creation of the purification table
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory144284
Lecture - Practice145298
Midterm Examination1426
Final Examination1426
TOTAL WORKLOAD (hours)194
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
5
4
4
3
4
OÇ-2
5
4
4
3
4
OÇ-3
5
4
4
3
4
OÇ-4
5
5
5
5
5
OÇ-5
5
5
5
4
4
Adnan Menderes University - Information Package / Course Catalogue
2026