Information Package / Course Catalogue
Proteomic Analysis
Course Code: ZBY537
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 3
Prt.: 0
Credit: 3
Lab: 0
ECTS: 6
Objectives of the Course

The aim of this course is to teach students the basic concepts of Proteomics, to have analytical thinking about proteins, to teach that the proteome is the proteins produced by a cell or organism under certain conditions, how these molecules are related to each other and how they form a biological system, and to teach the use of proteomics applications in other branches of science.

Course Content

Transition from Genomics to Proteomics, General Properties of Proteins, Separation techniques used in proteome analysis, Techniques used in the identification of proteins, Proteomics and analysis of protein sequences, Structural proteome analysis, Protein-protein interactions and proteomics, Protein modifications in proteome analysis, Protein chips and functional proteome analysis, Proteomics Applications

Name of Lecturer(s)
Learning Outcomes
1.Understand how proteins are interrelated and how they form a biological system
2.Learn that the proteome is the proteins produced by a cell or organism under certain conditions
3.Learning the principles of protein separation, identification and determination
4.Linking proteomics and its possible role in agricultural production
5.To be able to establish the connection between experimental logic, analytical thinking, quantitative analysis and problem solving
Recommended or Required Reading
1.Principles of Proteomics, RM Twyman, 2014, Garland Science, LCC
2.Proteomik, Münir Tuncer, 2022, Palme Yayınevi
3.Introduction To Proteomics: Principles and Applications -Nawin Mishra, 2010, John Wiley & Sons, Inc
4.Temel ve İleri Moleküler Biyoloji Yöntemleri-Genomik ve Proteomik Analizler. Editörler: Güler Temizkan, Nazlı Arda, 2018, Nobel Tıp Kitabevleri
Weekly Detailed Course Contents
Week 1 - Theoretical
Transition from Genomics to Proteomics, field of activity of Proteomics
Week 2 - Theoretical
General Properties of Proteins: Structure and function of proteins
Week 3 - Theoretical
Proteomics Tools and Analytical proteomics
Week 4 - Theoretical
Electrophoretic separation techniques in proteome analysis: SDS-PAGE and isoelectric focusing
Week 5 - Theoretical
Electrophoretic separation techniques in proteome analysis: Isoelectric focusing
Week 6 - Theoretical
Use of mass spectrometry in proteomics; protein identification using mass spectrometry information
Week 7 - Theoretical
Quantitative proteomics by standard two-dimensional gel and mass spectrometry (Midterm exam)
Week 8 - Theoretical
Chromatographic separation techniques in proteome analysis: High performance liquid chromatography
Week 9 - Theoretical
Chromatographic separation techniques in proteome analysis: Gel filtration chromatography
Week 10 - Theoretical
Chromatographic separation techniques in proteome analysis: Ion-exchange chromatography and affinity chromatography
Week 11 - Theoretical
Protein-protein interactions and proteomics: Principles of protein interaction analysis; Protein interaction maps
Week 12 - Theoretical
Structural proteome analysis: Importance of protein structures in proteomics; Techniques for understanding protein structures; Comparison of protein structures
Week 13 - Theoretical
Protein chips and functional proteome analysis
Week 14 - Theoretical
Proteomics Application Areas: Plant proteomics, pharmaceutical proteomics, disease diagnosis, drug and vaccine development
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory1473140
Midterm Examination1415
Final Examination1415
TOTAL WORKLOAD (hours)150
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
5
5
5
5
5
OÇ-2
4
4
4
5
5
OÇ-3
4
4
4
4
5
OÇ-4
4
4
4
4
5
OÇ-5
4
4
4
4
5
Adnan Menderes University - Information Package / Course Catalogue
2026