Week 1 - Theoretical
The importance of the microscope for investigations, the kinds of microscopes.
Week 1 - Preparation Work
Presentation of the electron microscope.
Week 2 - Theoretical
Thekinds of electronmicroscope, themechanism of electronmicroscope (TEM).
Week 2 - Preparation Work
Presentation of the electron microscope.
Week 3 - Theoretical
The preparation steps of TEM, the aim and the kinds of fixation, the rules of fixation , the aim of dehidratation and choosing of used materials.
Week 3 - Preparation Work
Removing tissues from organism.
Week 4 - Theoretical
The preparation steps of TEM, the aim and the kinds of fixation, the rules of fixation , the aim of dehidratation and, clearing, choosing of used materials. (Contuniation)
Week 4 - Preparation Work
Preperation of fixative.
Week 5 - Theoretical
The preparation steps of TEM; embedding materials, preparation of tissues for sectioning (trimming), sectioning, examining by microscope.
Week 5 - Preparation Work
Dehidration and clearing of tissues.
Week 6 - Theoretical
The preparation steps of TEM; embedding materials, preparation of tissues for sectioning (trimming), sectioning, examining by microscope. (Contuniation)
Week 6 - Preparation Work
The preparation of embedding materials.
Week 7 - Theoretical
Introduction of ultramicrotome and preparation for use, knife making.
Week 7 - Preparation Work
Presentation of the ultramicrotome and preparation for use.
Week 8 - Theoretical
Sectioning with ultramicrotome, preparation of blades. (Midterm)
Week 8 - Preparation Work
Preparation of blocks for sectioning. (Midterm)
Week 9 - Theoretical
Film coating on grids, sectioning process.
Week 9 - Preparation Work
Trimming, operation of the ultramicrotome, preparation of blades and grids, sectioning.
Week 10 - Theoretical
Trimming, preparation of blades and grids, sectioning, painting. (Contuniation)
Week 10 - Preparation Work
Trimming, preparation of blades and grids, sectioning, painting.
Week 11 - Theoretical
Staining of sections. (Contuniation)
Week 11 - Preparation Work
Trimming, preparation of blades and grids, sectioning, painting. (Contuniation)
Week 12 - Theoretical
Points to be taken into consideration during microscope examination, photography and evaluation.
Week 12 - Preparation Work
Examination under the microscope, to take photos.
Week 13 - Theoretical
Examination under the microscope, photography and evaluation. (Contuniation)
Week 13 - Preparation Work
Examination under the microscope, photography and evaluation.
Week 14 - Theoretical
Evaluation of achievements, repetition of information.