Information Package / Course Catalogue
| Course Code | : BİO500 |
| Course Type | : Area Elective |
| Couse Group | : Second Cycle (Master's Degree) |
| Education Language | : Turkish |
| Work Placement | : N/A |
| Theory | : 2 |
| Prt. | : 2 |
| Credit | : 3 |
| Lab | : 0 |
| ECTS | : 6 |
The aim of this course is to give information about the formation of micronucleus, micronucleus detection technique and micronucleus detection in buccal cells.
Micronucleus test is a useful bioassay, which to investigate the genotoxic and carcinogenic potentials of all kinds of chemical substances that we are frequently exposed to in everyday life such as physical agents, drugs, environmental pollutants and food additives in all plant, animal and human cells that have undergone mitosis. The increase in the frequency of occurrence of MN is considered as an indicator of damage to the genetic material.
| 1. | To be able to have knowledge about the structure and formation mechanism of micronuclei |
| 2. | Learns the environmental occupational risks that cause micronucleus formation |
| 3. | Learn the role of micronucleus in cell division kinetics |
| 4. | Define the techniques used in the determination of micronucleus |
| 5. | Learn buccal cells and how to determine micronuclei in buccal cells |
| 6. | Have information about the isolation of buccal cells, staining and how to count. |
| 7. | Learns the relationship between micronucleus frequency in buccal cells and cancer and other chronic diseases. |
| 1. | Şekeroğlu, v., Şekeroğlu-Atlı, Z. 2011. Genotoksik hasarın belirlenmesinde mikronükleus testi. Türk Hijyen ve Deneysel Biyoloji Dergisi. 68(4): 241-52. |
| 2. | Demirel, S., Gül Zamani.2002. Mikronükleus tekniği ve kullanım alanları. Genel Tıp Derg 12(3):123-127. |
| 3. | Edited by Siegfried Knasmüller , Edited by Michael Fenech. 2019. The Micronucleus Assay in Toxicology. Royal Society of Chemistry. 400 pp |
| Type of Assessment | Count | Percent |
|---|---|---|
| Practice | 2 | %10 |
| Assignment | 2 | %10 |
| Midterm Examination | 1 | %20 |
| Final Examination | 1 | %60 |
| Activities | Count | Preparation | Time | Total Work Load (hours) |
|---|---|---|---|---|
| Lecture - Theory | 14 | 1 | 2 | 42 |
| Lecture - Practice | 14 | 1 | 2 | 42 |
| Assignment | 1 | 1 | 2 | 3 |
| Laboratory | 5 | 0 | 5 | 25 |
| Individual Work | 10 | 0 | 2 | 20 |
| Midterm Examination | 1 | 15 | 1 | 16 |
| Final Examination | 1 | 2 | 1 | 3 |
| TOTAL WORKLOAD (hours) | 151 | |||
PÇ-1 | PÇ-2 | PÇ-3 | PÇ-4 | PÇ-5 | PÇ-6 | PÇ-7 | PÇ-8 | PÇ-9 | PÇ-10 | |
OÇ-1 | 1 | 4 | ||||||||
OÇ-2 | 4 | 3 | 3 | |||||||
OÇ-3 | 3 | 3 | 3 | 3 | ||||||
OÇ-4 | 3 | 3 | 3 | 2 | ||||||
OÇ-5 | 2 | 4 | 2 | 2 | ||||||
OÇ-6 | 2 | 3 | 2 | 2 | ||||||
OÇ-7 | 3 | 3 | 3 | |||||||