Information Package / Course Catalogue
Plant Cell and Tissue Culture
Course Code: BİO519
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 3
Prt.: 0
Credit: 3
Lab: 0
ECTS: 6
Objectives of the Course

Teaching of alternative plant propagation techniques by using plant cell and tissue culture methods.

Course Content

Cell and tissue culture, micropropagation, organogenesis, somatic embryogenesis, callus culture, somaclonal variation, haploid plant production, the production of disease-free plant

Name of Lecturer(s)
Learning Outcomes
1.Gain knowledge about basic concepts of totipotency, differentiation and plant cell and tissue culture.
2.Have knowledge of basic laboratory techniques regarding cell and tissue culture.
3.Gains knowledge about different cell and tissue culture technologies.
4.Researches and examines previous studies on course topics
5.Uses the knowledge gained within the scope of this course in solving problems in different disciplines.
Recommended or Required Reading
1.Plant tissue culture technology Betül Bürün, Ergun Kaya.2023. Nobel Akademic Publication 978-625-427-852-5
2.Plant Biotechnology Vol 1. Tissue Culture and its Applications Babaoğlu, M., Gürel, E., Özcan S.,S.Ü. Vakfı Yayınları, ISBN: 975-6652-04-7, (2001).
Weekly Detailed Course Contents
Week 1 - Theoretical
Totipotency and differentiation
Week 2 - Theoretical
Basic techniques in cell and tissue cultures
Week 2 - Preparation Work
Article examination
Week 3 - Theoretical
Micropropagation
Week 3 - Preparation Work
Article examination
Week 4 - Theoretical
Somatic embryogenesis and synthetic seed production
Week 4 - Preparation Work
Article examination
Week 5 - Theoretical
Meristem culture
Week 5 - Preparation Work
Article examination
Week 6 - Theoretical
Embryo culture
Week 6 - Preparation Work
Article examination
Week 7 - Theoretical
endosperm culture
Week 7 - Preparation Work
Article examination
Week 8 - Theoretical
Anther and microspore culture (Midterm exam)
Week 8 - Preparation Work
Article examination
Week 9 - Theoretical
Protoplast culture
Week 9 - Preparation Work
Article examination
Week 10 - Theoretical
Callus and cell suspansion culture
Week 10 - Preparation Work
Article examination
Week 11 - Theoretical
Secondary metabolite production
Week 11 - Preparation Work
Article examination
Week 12 - Theoretical
In vitro selection, somaclonal ve gametoclonal variations
Week 12 - Preparation Work
Article examination
Week 13 - Theoretical
Problems encountered in plant cell and tissue cultures
Week 13 - Preparation Work
Article examination
Week 14 - Theoretical
Data collection and evaluation in plant tissue culture studies
Week 14 - Preparation Work
Article examination
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory141356
Assignment33112
Individual Work141128
Midterm Examination120424
Final Examination128432
TOTAL WORKLOAD (hours)152
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
OÇ-1
1
3
3
3
4
5
5
OÇ-2
4
4
4
4
4
4
4
4
4
4
OÇ-3
OÇ-4
OÇ-5
Adnan Menderes University - Information Package / Course Catalogue
2026