Information Package / Course Catalogue
Enzyme Biochemistry
Course Code: BİO523
Course Type: Area Elective
Couse Group: Second Cycle (Master's Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 6
Objectives of the Course

To learn the properties of enzymes, catalysis mechanisms and activity determination methods.

Course Content

Structure, classification, catalytic mechanisms, kinetics, activation, inhibition and analysis methods of enzymes

Name of Lecturer(s)
Learning Outcomes
1.Learns and explains the structure of enzymes
2.Learns and explains the classification of enzymes
3.Learn and explain enzyme kinetics and catalysis mechanisms
4.Learn and explain the activation and inhibition of enzymes
5.Learns and applies measurement and kinetic characterization of enzyme activity
Recommended or Required Reading
1.Biyokimyanın Prensipleri (Lehninger), David L. Nelson & Michael M. Cox ; Nedret Kılıç, Palme Yayıncılık,2005
2.Biyokimya I, II, Engin Gözükara, Nobel Tıp Kitabevi, İstanbul, 1997
3.Enzymes, A Practical Introduction to Structure, Mechanism and Data Analysis, Robert A. Copeland, A. John Wiley and Sons Inc. Publication, 2000
4.Enzyme Chemistry Impact and applications, C. J. Suckling, C. L. Gibson, A. R. Pitt, Blackie Academic and Professional, 1998.
Weekly Detailed Course Contents
Week 1 - Theoretical
Common features of enzymes
Week 1 - Preparation Work
Enzyme purification
Week 2 - Theoretical
Classification of enzymes
Week 2 - Preparation Work
Enzyme purification
Week 3 - Theoretical
Enzymatic catalyst mechanism
Week 3 - Preparation Work
Enzyme purification
Week 4 - Theoretical
Enzyme kinetics
Week 4 - Preparation Work
Enzyme activity assays
Week 5 - Theoretical
Factors influencing enzyme activity
Week 5 - Preparation Work
Factors influencing enzyme activity(pH and temperature)
Week 6 - Theoretical
Inhibition of enzymes
Week 6 - Preparation Work
Factors influencing enzyme activity(pH and temperature stability)
Week 7 - Theoretical
Allosteric enzymes
Week 7 - Preparation Work
Factors influencing enzyme activity(Substrate concentration)
Week 8 - Theoretical
Allosteric enzymes
Week 8 - Preparation Work
Factors influencing enzyme activity(Substrate concentration)
Week 9 - Theoretical & Practice
Enzyme temperature and pH stability determinations
Week 10 - Theoretical
Enzyme specificity
Week 10 - Preparation Work
Enzyme specificity
Week 11 - Theoretical
Enzymatic catalyst mechanism
Week 11 - Preparation Work
Enzymatic catalyst mechanism
Week 12 - Theoretical
Regulation of enzyme activity
Week 12 - Preparation Work
Regulation of enzyme activity
Week 13 - Theoretical
Determination of enzyme activity, spectrofotometric applications.
Week 13 - Preparation Work
Determination of enzyme activity, spectrofotometric applications.
Week 14 - Theoretical
Structure and function of coenzymes.
Week 14 - Preparation Work
Structure and function of coenzymes.
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory141242
Lecture - Practice141242
Assignment141128
Individual Work141128
Midterm Examination1516
Final Examination1516
TOTAL WORKLOAD (hours)152
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
OÇ-1
5
5
5
5
5
5
4
4
3
3
OÇ-2
5
4
5
5
5
3
5
3
3
2
OÇ-3
2
3
2
1
5
2
5
3
2
OÇ-4
5
5
5
2
2
5
5
5
5
OÇ-5
Adnan Menderes University - Information Package / Course Catalogue
2026