Information Package / Course Catalogue - Adnan Menderes University

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Bacterial Identification Methods

Course Code	: BİO555
Course Type	: Area Elective
Couse Group	: Second Cycle (Master's Degree)
Education Language	: Turkish
Work Placement	: N/A

Theory	: 3
Prt.	: 0
Credit	: 3
Lab	: 0
ECTS	: 6

Objectives of the Course

The characterization of a strain is a key element in prokaryote systematics. Both classical phenotypic and molecular methods are used in bacteria identification. The aim of this course is to teach methods used in bacterial identification.

Course Content

Systematics of Bacteria, species concept in bacteriology, morphological and biochemical methods used in bacterial identification, PCR based methods, DNA-DNA hybridization, rapid identification test kits, identification and nomenclature of new species

Name of Lecturer(s)

Learning Outcomes

1.	To be able to describe bacterial species concept
2.	To be able to recognize current status in bacteria systematics
3.	To be able to recognize morphological methods and their principles used in bacteria identification
4.	To be able to cognize biochemical methods and their principles used in bacteria identification
5.	To be able to cognize molecular methods and their principles used in bacteria identification
6.	To be able to comprehend characterization and publication of new bacteria species
7.	To be able to cognize rapid identification test kits in bacteria identification
8.	To be able to understand culture independent methods in microbial systematics

Recommended or Required Reading

1.	Bergey’s Manual of Determinative Bacteriology
2.	Bergey’s Manual of Systematic Bacteriology
3.	Methods for general and molecular bacteriology

Weekly Detailed Course Contents

Week 1 - Theoretical

Bacterial species concept and Bergey’s Manual of Systematic Bacteriology

Week 2 - Theoretical

Diversity of bacterial species

Week 3 - Theoretical

Morphological Methods used in Bacterial Identification 1

Week 4 - Theoretical

Morphological Methods used in Bacterial Identification 2

Week 5 - Theoretical

Cultural Methods used in Bacterial Identification

Week 6 - Theoretical

Biochemical Tests used in Bacterial Identification

Week 7 - Theoretical

Serological Tests used in Bacterial Identification

Week 8 - Theoretical & Practice

Identification of Gram positive and Gram negative bacterial members (Midterm exam)

Week 9 - Theoretical

Molecular Methods used in Bacterial Identification (PCR based methods)

Week 10 - Theoretical

Molecular Methods used in Bacterial Identification (DNA-DNA hybridization)

Week 11 - Theoretical

Publication of new species

Week 12 - Theoretical

Currend status in Bacterial Systematics

Week 13 - Theoretical

Rapid Identification Methods 1

Week 14 - Theoretical

Rapid Identification Methods 2

Assessment Methods and Criteria

Type of Assessment	Count	Percent
Midterm Examination	1	%30
Final Examination	1	%70

Workload Calculation

Activities	Count	Preparation	Time	Total Work Load (hours)
Lecture - Theory	14	0	3	42
Laboratory	1	0	4	4
Reading	14	0	3	42
Individual Work	14	0	2	28
Midterm Examination	1	10	1	11
Final Examination	1	20	1	21
TOTAL WORKLOAD (hours)				148

Contribution of Learning Outcomes to Programme Outcomes

	PÇ-1	PÇ-2	PÇ-3	PÇ-4	PÇ-5	PÇ-6	PÇ-7	PÇ-8	PÇ-9	PÇ-10
OÇ-1
OÇ-2
OÇ-3	2	4	1	3	4	2	4	2	4	2
OÇ-4
OÇ-5
OÇ-6	2	4	2	4		5	3	4	5	1
OÇ-7
OÇ-8

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