Information Package / Course Catalogue
Genetic Polymorphism and Polymorphism Determination Methods
Course Code: ZBY603
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

The aim of this course is to give the students the knowledge the sources of variation in DNA and to learn how to use this information in experimental research.

Course Content

A new era began with the inventions of polymerase chain reaction in molecular genetics. In this course the variation sources, polymorphism and mutation separation, basics of PCR technique, design of simple PCR reactions, indirect mutation analysis techniques, direct mutation analysis techniques, fragment analysis for phylogenetic studies, Real- Time PCR technique will be explained. The student will learn the localization and structure of the genes of interest by using genome databases, be able to design primers for PCR and / or RT- PCR, and where to start designing genetic experiments for their own research.

Name of Lecturer(s)
Learning Outcomes
1.Student will learn how to ues genomic databases to find the localizations of the genes
2.Student will learn how to design a primary
3.Within the scope of the course Students will learn the techniques of DNA sequencing and gene expression analysis.
4.Learns gen expression analysis
5.Learns mutation analysis
Recommended or Required Reading
1.Genome: The Autobiography of a Species in 23 Chapters, Matt Ridley
2.GENOMLAR 3, T.A. Brown, Nobel Akademik Yayıncılık
3.Principles of Gene Manipulation and Genomics, 7th Edition. S.B. Primrose and R.M. Twyman. Blackwell Publishing, 2006.
4.Klug, W. S., Cummings, M. R., Spencer, C. A., Palladino, M. A., & Ward, S. M. (2009). Concepts of genetics (pp. 463-464). Pearson
5.Kwok PY. Methods in Molecular Biology “Single Nucleotide Polymorphisms Methods and Protocols”, 2003, Humana Press
6.O’Connell J. Methods in Molecular Biology “RT-PCR Protocols”, 2002, Humana Press
Weekly Detailed Course Contents
Week 1 - Theoretical & Practice
The design of genetic analysis experiments
Week 2 - Theoretical & Practice
The determination of a gene structure using bioinformatic tools
Week 3 - Theoretical & Practice
The determination of a gene structure using bioinformatic tools- contiues
Week 4 - Theoretical & Practice
Primer design
Week 5 - Theoretical & Practice
PCR fundamentals
Week 6 - Theoretical & Practice
PCR optimization
Week 7 - Theoretical & Practice
PCR optimization
Week 8 - Theoretical & Practice
Mutation and mutation analysis approaches
Week 9 - Theoretical & Practice
Mutation and mutation analysis approaches
Week 10 - Theoretical & Practice
Indirect mutation analyses approaches
Week 11 - Theoretical & Practice
Direct mutation analysis approaches
Week 12 - Theoretical & Practice
Sequence comparison and alignment using bioinformatics analysis tools
Week 13 - Theoretical & Practice
Repetitive DNA elements and their role in ID determination and phylogenetic analysis
Week 14 - Theoretical & Practice
Gene Expression Analysis, Real Time PCR: gene expression analysis
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory144284
Lecture - Practice144284
Midterm Examination112113
Final Examination112113
TOTAL WORKLOAD (hours)194
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
5
5
5
5
5
OÇ-2
4
4
4
3
4
OÇ-3
4
3
3
4
4
OÇ-4
4
5
5
3
3
OÇ-5
3
4
4
3
4
Adnan Menderes University - Information Package / Course Catalogue
2026