Information Package / Course Catalogue
Molecular Biotechnology Applications
Course Code: ZBY637
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

The aim of Molecular Biotechnology Applications course is to teach the students the doctorate in biotechnology, to teach the theoretical basis of molecular biology-based experimental applications, to teach them how to practice and to make DNA manipulation techniques.

Course Content

DNA isolation and characterization, Restrictional digestion, restrictional length polymorphism, DNA ligase applications, cohesive and blunt end ligation, transformation, Polymerase chain reaction (PCR) and The parameters affecting the reliability of PCR, Preparation and screening of genomic and cDNA libraries, Footprint analyses, Southern Blot, Reverse transcriptase polymerase chain reaction, in vitro mutagenesi, DNA sequencing, Patenting Biotechnology Inventions and Ethical Considerations

Name of Lecturer(s)
Learning Outcomes
1.İmprovement of personal capability on experimental biotechnology applications
2.Comprehension of basic molecular biotechnology processes.
3.Learning laboratory processes in molecular biology and biotechnology
4.Designing a biotechnology-oriented project in agricultural activities
5.Interrelated and comparative academic thinking and interpretation among the fields of Agricultural Engineering, Agricultural Biotechnology, Genetic Engineering, Genetics, Molecular Biology and Biotechnology.
Recommended or Required Reading
1.Watson JD, Baker, T.A., Bell, S.P., Gann, A., Levine, M.,Losick, R. 2007. Molecular Biology of the Gene, 6th. Ed., Pearson Benjamin Cummings Publishing Company ve Cold Spring Harbour Laboratory Press, New York
2.Snyder, L., Champness W., 2007, Molecular Genetics of Bacteria (3th Edition),ASM Press , Washington
Weekly Detailed Course Contents
Week 1 - Theoretical & Practice
DNA isolation and characterization
Week 2 - Theoretical & Practice
Restrictional digestion, restrictional length polymorphism
Week 3 - Theoretical & Practice
DNA ligase applications, cohesive and blunt end ligation, transformation
Week 4 - Theoretical & Practice
Polymerase chain reaction (PCR) and The parameters affecting the reliability of PCR
Week 5 - Theoretical & Practice
Preparation and screening of genomic and cDNA libraries
Week 6 - Theoretical & Practice
Electroforetic mobility shift measurements
Week 7 - Theoretical & Practice
Footprint analyses, Southern Blot, Reverse transcriptase polymerase chain reaction, in vitro mutagenesi, DNA sequencing, Patenting Biotechnology Inventions and Ethical Considerations
Week 8 - Theoretical & Practice
Southern Blot
Week 9 - Theoretical & Practice
Reverse transcriptase polymerase chain reaction
Week 10 - Theoretical & Practice
In vitro mutagenesis
Week 11 - Theoretical & Practice
DNA sequencing
Week 12 - Theoretical & Practice
Patenting Biotechnology Inventions and Ethical Considerations
Week 13 - Theoretical & Practice
Reviews on the evaluation and commercial applications of biotechnological publications-1
Week 14 - Theoretical & Practice
Reviews on the evaluation and commercial applications of biotechnological publications-2
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory78163
Lecture - Practice78163
Assignment510155
Midterm Examination110111
Final Examination110111
TOTAL WORKLOAD (hours)203
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
5
5
5
4
5
OÇ-2
5
5
5
4
5
OÇ-3
5
4
5
4
5
OÇ-4
5
4
4
5
4
OÇ-5
4
4
5
5
4
Adnan Menderes University - Information Package / Course Catalogue
2026