The objective of this course is to get the students to comprehend the principles of the main techniques used in genetic and protein engineering /molecular biotechnology and to introduce the recent technologies.

r-DNA technologies, short history of the genetic and protein engineering, Isolation of the DNA and RNA, electrophoresis of the nucleic acids, Hybridization of the nucleic acids, Southern and Northern Blot analysis, The use of enzymes in the modification of nucleic acids,Restriction enzymes,Polimerase chain reaction and its applications,DNA sequencing,Midterm exam,Recombinant protein expression, expression systems (vectors and hosts), homologue and heterologue gene expression aproaches, Genom and proteom analysis, functional geneomics, techniques to determine protein-protein and protein-DNA interactions,Protein isolation and purification, electrophoretic and chromatographic techniques, Western Blot analysis,Aproaches used in protein engineering: rational design and site directed mutagenesis, directed evolution and library creation, Molecular diagnostics in medicine, techniques used in drug research and industry, Molecular based techniques used in determination and also characterization of the biodiversity