Information Package / Course Catalogue
Experimental Biochemistry
Course Code: ZBY609
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

Within the scope of this course, it is aimed to teach various biochemical methods with theoretically and practically used in biochemistry laboratory and research.

Course Content

Spectroscopic, electrophoretic and chromatographic techniques will be explained and experimental studies will be made using these techniques.

Name of Lecturer(s)
Learning Outcomes
1.Have theoretical knowledge about biochemical methods.
2.Learns sample preparation techniques.
3.Learns the analysis of biomolecules by spectrophotometric method
4.Gains knowledge about purification of proteins by chromatographic techniques
5.Learns the imaging and characterization of proteins by electrophoretic techniques
Recommended or Required Reading
1.Methods used in molecular biology, 3.edition, Nobel tıp kitabevleri 2008, Editörler; Prof.Dr. Güler Temizkan, Prof. Dr.Nazlı Arda
2.Modern Experimental Biochemistry 3rd edition, Published by Pearson, 2001, Rodney F. Boyer
Weekly Detailed Course Contents
Week 1 - Theoretical & Practice
Preparation and storege of solutions
Week 2 - Theoretical & Practice
Theory and practice of pH and buffer solutions
Week 3 - Theoretical & Practice
Techniques for sample preparations (dialysis, ultrafiltration,lyophilization)
Week 4 - Theoretical & Practice
Protein determination methods
Week 5 - Theoretical & Practice
Measurement of protein an nucleic acid samples by spectroscopic method
Week 6 - Theoretical & Practice
Chromatographic techniques (paper and thin layer chromatography)
Week 7 - Theoretical & Practice
Chromatographic techniques (column chromatography)
Week 8 - Theoretical & Practice
Chromatographic techniques (column chromatography)
Week 9 - Theoretical & Practice
Determination of enzyme activity spectrophotometrically
Week 10 - Theoretical & Practice
Electrophoretic methods
Week 11 - Theoretical & Practice
Screening of proteins by electrophoresis (SDS-PAGE)
Week 12 - Theoretical & Practice
Native gelelectrophoresis (PAGE) and zymogram
Week 13 - Theoretical & Practice
Total lipid and fatty acid determination from natural oils
Week 14 - Theoretical & Practice
Determination of glycogen from animal tissues
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory145298
Lecture - Practice145298
Midterm Examination1325
Final Examination1325
TOTAL WORKLOAD (hours)206
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
5
4
4
OÇ-2
5
4
3
OÇ-3
5
5
3
3
OÇ-4
5
5
4
3
3
OÇ-5
5
5
3
3
Adnan Menderes University - Information Package / Course Catalogue
2026