Information Package / Course Catalogue
Laboratory Techniques in Microbiology
Course Code: ZBY627
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 0
Credit: 3
Lab: 2
ECTS: 8
Objectives of the Course

The course aims to enable students to gain practical knowledge and skills about biochemical tests, cultivation, isolation, and inoculation methods used in fungi and bacteria in the field of microbiology and to plan their studies for their theses.

Course Content

Bacteria and fungi recognition, isolation, identification, and storage.

Name of Lecturer(s)
Learning Outcomes
1.Recognise plant pathogens
2.Can do microbiological studies in the laboratory
3.Knows the differences between bacteria and fungi
4.Can store bacteria and fungi for a long time
5.Have knowledge about microbial physiology
Recommended or Required Reading
1.Petersen J, McLaughlin S. 2016: Laboratory Exercises in Microbiology: Discovering the Unseen World Through Hands-On Investigation. City University of New York (CUNY)
2.Schaad, N. W. J. B. Jones, W. Chun, Laboratory Guide for Identification of Plant Pathogenic Bacteria, APS Press,St. Paul, Minnesota, ISBN-0-89054-263-5
3.Iren S. and Z. Katırcıoğlu, 1989. Applied Mycology. Ankara University Faculty of Agriculture Publications Ankara No: 1126. p. 136
Weekly Detailed Course Contents
Week 1 - Theoretical & Practice
Characteristics of plant and animal pathogenic bacteria and fungi / rules to be followed in the laboratory
Week 2 - Theoretical & Practice
Introduction and contents of media/ Preparation of general and selective media
Week 3 - Theoretical & Practice
Isolation of bacteria and fungi from diseased material/ Isolation and colony morphology examination
Week 4 - Theoretical & Practice
Microbial counting methods /Dilution, Spectrophotometric methods
Week 5 - Theoretical & Practice
Methods of obtaining pure culture /Inoculum preparation
Week 6 - Theoretical & Practice
Spore concentration calculation by haemocytometer / microscopic counting
Week 7 - Theoretical & Practice
Differences between spores formed in bacteria and moulds / Endospore staining, mould spore staining
Week 8 - Theoretical & Practice
Soil sampling methods
Week 9 - Theoretical & Practice
Determination of bacterial count in soil sample/ determination of bacterial count in CFU/ML
Week 10 - Theoretical & Practice
Microscopic examination of bacteria and moulds / Gram Staining, Mould Staining
Week 11 - Theoretical & Practice
Biochemical tests / Oxidase, catalase, plant hormone production tests
Week 12 - Theoretical & Practice
Pathogenicity tests /Inoculation to plants
Week 13 - Theoretical & Practice
Long-term preservation methods of bacteria and fungi / storage of isolates at -80 0C
Week 14 - Theoretical & Practice
Lyophilisation techniques
Assessment Methods and Criteria
Type of AssessmentCountPercent
Attending Lectures1%5
Laboratory1%5
Midterm Examination1%20
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory144284
Laboratory145298
Midterm Examination1819
Final Examination1819
TOTAL WORKLOAD (hours)200
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
4
4
3
5
5
OÇ-2
5
3
4
5
5
OÇ-3
3
3
3
3
5
OÇ-4
3
4
4
5
5
OÇ-5
3
4
5
5
5
Adnan Menderes University - Information Package / Course Catalogue
2026