Information Package / Course Catalogue
DNA Barcode Techniques
Course Code: ZBY639
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 0
Credit: 3
Lab: 2
ECTS: 8
Objectives of the Course

The aim is to learn and analyse the genes used to barcode eukaryotes and prokaryotes, and to use them in molecular diagnostics.

Course Content

In eukaryotes, the nucleus, chloroplast and mitochondria; in prokaryotes, the use of molecular diagnostic genes, bioinformatics tools for DNA barcode analysis and uploading of analysed genes to NCBI.

Name of Lecturer(s)
Learning Outcomes
1.Learn the concept of DNA barcoding
2.Learn how to use DNA barcoding analysis
3.Learn the importance of molecular diagnostic techniques
4.Learn about DNA barcode regions in prokaryotes
5.Learning DNA barcode regions in eukaryotes and the use of bioinformatics programs in DNA barcode analysis
Recommended or Required Reading
1.Molecular Biology, Nobel Academic Publishing Consulting Trade. Professor Dr. Mehmet Karataş, 2012, Nei M, Kumar S (2000) Molecular evolution and phylogenetics. Oxford University press
2.Fundamentals of Molecular Genetics, Assoc. Prof. Dr. H. Ümit LÜLEYAP, Nobel Kitapevi, 2008, Bitki Biyoteknolojisi (Genetik Mühendisliği ve Uygulamaları) Sebahattin Özcan, Ekrem Gürel, Mehmet Babaoğlu. Selçuk Üniversitesi Vakfı Yayınları
Weekly Detailed Course Contents
Week 1 - Theoretical
Structure and replication of the DNA molecule
Week 1 - Laboratory
Use of DNA isolation protocols
Week 2 - Theoretical
Discussion of the DNA barcode concept
Week 2 - Laboratory
PCR and Electrophoresis
Week 3 - Theoretical
Molecular diagnostics in prokaryotes
Week 3 - Laboratory
Barcoding regions amplified in prokaryotes
Week 4 - Theoretical
Use of nuclear barcode genes
Week 4 - Laboratory
Amplification of nrDNA barcode regions
Week 5 - Theoretical
Use of chloroplast barcode genes I
Week 5 - Laboratory
Amplifying chloroplast barcoded regions
Week 6 - Theoretical
Use of chloroplast barcode genes II
Week 6 - Laboratory
Amplifying chloroplast barcoded regions
Week 7 - Theoretical
Use of mitochondrial barcode genes
Week 7 - Laboratory
Amplifying mitochondria barcoded regions
Week 8 - Theoretical
Use of mitochondrial barcode genes (Midterm Exam)
Week 8 - Laboratory
Mitokondri barkod genlerinin kullanımı (Ara Sınav)
Week 9 - Theoretical
Use of barcode genes in fungi
Week 9 - Laboratory
Amplification of fungal barcode regions
Week 10 - Theoretical
Processing of DNA barcode genes
Week 10 - Laboratory
Implementing NCBI, Blast, Sequencher, BioEdit, FinchTV, Chromas, Codon Code Aligner, tree-seq and DNASP.
Week 11 - Theoretical
Use of bioinformatic tools for analysis of DNA barcode genes I
Week 11 - Laboratory
Implementing NCBI, Blast, Sequencher, BioEdit, FinchTV, Chromas, Codon Code Aligner, tree-seq and DNASP.
Week 12 - Theoretical
Use of bioinformatic tools for analysis of DNA barcode genes II
Week 12 - Laboratory
Implementing NCBI, Blast, Sequencher, BioEdit, FinchTV, Chromas, Codon Code Aligner, tree-seq and DNASP.
Week 13 - Theoretical
Use of DNA barcode genes in phylogenetic analysis
Week 13 - Laboratory
Phylogenetic analysis methods
Week 14 - Theoretical
Uploading DNA barcode genes to NCBI
Week 14 - Laboratory
Converting and analysing exon gene regions into protein sequences.
Week 31 - Preparation Work
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Rate1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory145298
Laboratory144284
Midterm Examination1516
Final Examination1516
TOTAL WORKLOAD (hours)194
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
OÇ-1
4
4
4
4
4
OÇ-2
4
5
5
5
5
OÇ-3
5
5
5
5
5
OÇ-4
4
4
4
4
5
OÇ-5
4
4
4
4
5
Adnan Menderes University - Information Package / Course Catalogue
2026