Information Package / Course Catalogue
Course Code
Course Type
Couse Group
Education Language
Work Placement
Theory: 0
Prt.: 0
Credit: 0
Lab: 0
ECTS
Objectives of the Course

Course Content

Name of Lecturer(s)
Learning Outcomes
1.
2.
3.
4.
5.
Recommended or Required Reading
1.Using and evaluating serological (double diffusion, immunofluorescence, ELISA) and molecular (PCR, BioPCR, RepPCR, RealtimePCR, qPCR) diagnostic methods that allow the identification and identification of plant pathogen prokaryotes from pure cultures, with or without indication
2.Schaad, N. W. J. B. Jones, W. Chun, Laboratory Guide for Identification of Plant Pathogenic Bacteria, APS Press, St. Paul, Minnesota, ISBN-0-89054-263-5
3.Maniatis, T. A., E. F. Fritsch, J. Sambrook, 1982. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, NY.
Weekly Detailed Course Contents
Week 1 - Theoretical & Practice
Use of diagnostic and identification tests for Phytopathogenic prokaryotes
Week 2 - Theoretical & Practice
Serology
Week 3 - Theoretical & Practice
Classical serological tests (Double diffusion, slide agglutination)
Week 4 - Theoretical & Practice
Immunofluorescens
Week 5 - Theoretical & Practice
ELISA
Week 6 - Theoretical & Practice
Immunoelectrophoresis
Week 7 - Theoretical & Practice
Bacteriophages and diagnostic assays
Week 8 - Theoretical & Practice
Molecular techniques
Week 9 - Theoretical & Practice
Molecular techniques
Week 10 - Theoretical & Practice
Polymerase Chain Reaction
Week 11 - Theoretical & Practice
Rep, PCR, Box-PCR, BioPCR
Week 12 - Theoretical & Practice
RealTime PCR
Week 13 - Theoretical & Practice
RealTime PCR
Week 14 - Theoretical & Practice
Advantages and disadvantages of assays and their practial uses
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory134278
Assignment210224
Laboratory132252
Midterm Examination122123
Final Examination122123
TOTAL WORKLOAD (hours)200
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
OÇ-1
4
3
4
5
5
5
4
OÇ-2
4
3
4
5
5
5
4
OÇ-3
4
3
4
5
5
5
4
OÇ-4
4
3
4
5
5
5
4
OÇ-5
4
3
4
5
5
5
4
Adnan Menderes University - Information Package / Course Catalogue
2026