Information Package / Course Catalogue
Molecular Plant Pathology
Course Code: ZBK607
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 3
Prt.: 0
Credit: 3
Lab: 0
ECTS: 6
Objectives of the Course

This course will explore the modern molecular techniques being used to investigate plant/microbe interactions and will examine the way in which pathogens cause disease.

Course Content

The contents encompass the modern molecular techniques in plant pathology. Genom organization, genetic of the host-pathogen interaction, pathogenic determinants are defined. Identification of by RNA extraction-isolation, northern blot analysis DNA extraction-isolation, southern blot analysis DNA/RNA hybridization, dot-blot hybridization. Labelling and storage of nucleic asid probes. Nucleic acid amplification tecniques: polymerase chain reaction. Genome analysis by RFLP and RAPD, cloning and gene expression. Characterized defence response genes. Genetic modification of plants for pathogens resistance are discussed.

Name of Lecturer(s)
Learning Outcomes
1.Acceptance of basic knowledge on most important molecular techniques used in plant pathology.
2.Achieving of skills important for decision making on electing of techniques needed to be performed in order to diagnose important plant pathogens
3.Capability development in problem analysis and synthesis in plant pathogen molecular diagnostics.
4.Achieving of skills important for decision making on electing of techniques needed to be performed in order to diagnose important plant pathogens
5.Capability development in problem analysis and synthesis in plant pathogen molecular diagnostics.
Recommended or Required Reading
1.Goosen, T. And Debets, 1996. Molecular genetic analysis. In: Fungal Genetic: Principles and practce, Bos, C.J. (ed.) Marcel Dekker, New York, pp.97-117.
2.Berger S.L. Guide to Molecular Cloning Techniques Academic Pres
3.Tower K.J. and A. Cockayne 1993. Molecular Methods for Microbial Identification and Typing. Chapman and Hall
4.Gurr S.J. and D.J. Bowles 1992 Molecular Plant Pathology Volume I, II,A Practical Approarh, At Oxfort Univ.Press
Weekly Detailed Course Contents
Week 1 - Theoretical
Causal agents of plant diseases: Pathogens
Week 2 - Theoretical
Reproductive biology of pathogens
Week 3 - Theoretical
Genome organization, Extrachromosomal inheritance
Week 4 - Theoretical
Genetic of the host-pathogen interaction
Week 5 - Theoretical
Gene transformation in pathogens
Week 6 - Theoretical
Genetic variation
Week 7 - Theoretical
Molecular markers
Week 8 - Theoretical
Molecular markers
Week 9 - Theoretical
Identification of pathogens: Nucleic acid izolation and hybridization techniques
Week 10 - Theoretical
Dot-blot hybridization, Gel electrophorosis, Electroelition
Week 11 - Theoretical
Southern, Northern blot hybridization
Week 12 - Theoretical
Identification of immunological materyal
Week 13 - Theoretical
Protein isolation and electrophorosis
Week 14 - Theoretical
Western blot hybridization
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory142256
Assignment220244
Quiz1718
Midterm Examination120121
Final Examination120121
TOTAL WORKLOAD (hours)150
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
OÇ-1
5
4
3
1
3
1
2
OÇ-2
4
4
3
1
3
1
2
OÇ-3
4
4
3
1
3
1
2
OÇ-4
OÇ-5
Adnan Menderes University - Information Package / Course Catalogue
2026