Information Package / Course Catalogue
Modern Diagnostic Techniques in Phytobacteriology
Course Code: ZBK622
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 6
Objectives of the Course

Painting methods for the examination of fungi growth in plant tissues

Course Content

Using and evaluating serological (double diffusion, immunofluorescence, ELISA) and molecular (PCR, BioPCR, RepPCR, RealtimePCR, qPCR) diagnostic methods that allow the identification and identification of plant pathogen prokaryotes from pure cultures, with or without indication

Name of Lecturer(s)
Learning Outcomes
1.
2.
3.
4.
5.
Recommended or Required Reading
1.Using and evaluating serological (double diffusion, immunofluorescence, ELISA) and molecular (PCR, BioPCR, RepPCR, RealtimePCR, qPCR) diagnostic methods that allow the identification and identification of plant pathogen prokaryotes from pure cultures, with or without indication
2.Schaad, N. W. J. B. Jones, W. Chun, Laboratory Guide for Identification of Plant Pathogenic Bacteria, APS Press, St. Paul, Minnesota, ISBN-0-89054-263-5
3.Maniatis, T. A., E. F. Fritsch, J. Sambrook, 1982. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, NY.
Weekly Detailed Course Contents
Week 1 - Theoretical & Practice
Use of diagnostic and identification tests for Phytopathogenic prokaryotes
Week 2 - Theoretical & Practice
Serology
Week 3 - Theoretical & Practice
Classical serological tests (Double diffusion, slide agglutination)
Week 4 - Theoretical & Practice
Immunofluorescens
Week 5 - Theoretical & Practice
ELISA
Week 6 - Theoretical & Practice
Immunoelectrophoresis
Week 7 - Theoretical & Practice
Bacteriophages and diagnostic assays
Week 8 - Theoretical & Practice
Molecular techniques
Week 9 - Theoretical & Practice
Molecular techniques
Week 10 - Theoretical & Practice
Polymerase Chain Reaction
Week 11 - Theoretical & Practice
Rep, PCR, Box-PCR, BioPCR
Week 12 - Theoretical & Practice
RealTime PCR
Week 13 - Theoretical & Practice
RealTime PCR
Week 14 - Theoretical & Practice
Advantages and disadvantages of assays and their practial uses
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory133265
Assignment210224
Laboratory131239
Midterm Examination110111
Final Examination110111
TOTAL WORKLOAD (hours)150
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
OÇ-1
4
3
4
5
5
5
4
OÇ-2
4
3
4
5
5
5
4
OÇ-3
4
3
4
5
5
5
4
OÇ-4
4
3
4
5
5
5
4
OÇ-5
4
3
4
5
5
5
4
Adnan Menderes University - Information Package / Course Catalogue
2026