The aim of this course is to provide information about High Performance Liquid Chromatography.

The importance of modern liquid chromatography in other chromatographic methods and the place of HPLC amongst the analytical methods are introduced. The modes of liquid chromatography (adsorption, partition, ion-exchange and size exclusion) are presented in detail. Instrumentation of HPLC is introduced in real laboratory conditions. The applications of liquid chromatographic analysis in biomedical, clinical, environmental, food pharmaceutical areas are examined.