Information Package / Course Catalogue
Liquid Chjromatography
Course Code: KİM611
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 3
Prt.: 0
Credit: 3
Lab: 0
ECTS: 8
Objectives of the Course

The aim of this course is to provide information about High Performance Liquid Chromatography.

Course Content

The importance of modern liquid chromatography in other chromatographic methods and the place of HPLC amongst the analytical methods are introduced. The modes of liquid chromatography (adsorption, partition, ion-exchange and size exclusion) are presented in detail. Instrumentation of HPLC is introduced in real laboratory conditions. The applications of liquid chromatographic analysis in biomedical, clinical, environmental, food pharmaceutical areas are examined.

Name of Lecturer(s)
Learning Outcomes
1.To review fundamental knowledge on chromatographic equilibrium and rate theories.
2.To discuss and apprehend the place and significance of liquid chromatography among other analytical technics.
3.To apprehend theoritical principles of liquid chromatographic seperation modes.
4.To learn the principles of normal and reverse phase chromatography and discuss practical applications.
5.To get acquainted with liquid chromatographic instrumentation.
Recommended or Required Reading
1.High Performance Liquid Chromatography. Fundamental Principles and Practice. Eds: W.J. Lough, I.W. Wainer. Chapman & Hall, 1996
2.Practical High Performance Liquid Chromatography. V.R. Meyer. Wiley- VCH, 2004
Weekly Detailed Course Contents
Week 1 - Theoretical
HPLC versus other analytical methods
Week 2 - Theoretical
Efficiency, retention, selectivity and resolution
Week 3 - Theoretical
Modes of chromatography. Partition and adsorption
Week 4 - Theoretical
Modes of chromatography. Ion exchange and size exclusion
Week 5 - Theoretical
Normal and reverse phase chromatography
Week 6 - Theoretical
Support materials and solvents
Week 7 - Theoretical
Instrumentation. Pumps, injectors
Week 8 - Theoretical
Instrumentation. Column design
Week 9 - Theoretical
Instrumentation. Detectors
Week 10 - Theoretical
Method development and quantitation
Week 11 - Theoretical
Sample preparation
Week 12 - Theoretical
HPLC in biomedical and clinical analysis
Week 13 - Theoretical
HPLC in environmental analysis
Week 14 - Theoretical
HPLC in food and pharmaceutical analysis
Assessment Methods and Criteria
Type of AssessmentCountPercent
Midterm Examination1%30
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory141356
Assignment325075
Midterm Examination125227
Final Examination135237
TOTAL WORKLOAD (hours)195
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
OÇ-1
4
3
3
2
OÇ-2
4
3
3
2
OÇ-3
4
3
3
2
OÇ-4
4
3
3
2
OÇ-5
4
3
3
2
Adnan Menderes University - Information Package / Course Catalogue
2026