Information Package / Course Catalogue
Techniques of Protein Denaturation
Course Code: VBY631
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 1
Prt.: 2
Credit: 2
Lab: 0
ECTS: 4
Objectives of the Course

To explain the principle of protein denaturation, teach the methods of denaturing,

Course Content

The principle of protein denaturation, precipitation of proteins by heat, alcohol, acid, ammonium sulfate, and salts of heavy metals

Name of Lecturer(s)
Learning Outcomes
1.Learn the structure of proteins
2.Learn the methods of protein denaturation
3.Gain the ability to analyze and make hypothesis
4.To learn the chemical properties of proteins
5.To learn the methods of precipitation of proteins
Recommended or Required Reading
1.1-Klinik biyokimya (Yüregir, Güneş T.),
2.2- Klinik biyokimya = clinical biochemistry for medical students(Laker, M. F.),
3.3-Klinik biyokimya analiz metodları (Adam, Bahattin)
Weekly Detailed Course Contents
Week 1 - Theoretical
Definition of proteins
Week 1 - Practice
The introduction of laboratory equipment
Week 2 - Theoretical
Fuctions of protein
Week 2 - Practice
The preparation of work plan
Week 3 - Theoretical
Chemical structure of proteins
Week 3 - Practice
Preparation of tools and equipment
Week 4 - Theoretical
Simple proteins
Week 4 - Practice
Precipitation with heat test proteins
Week 5 - Theoretical
Conjugated proteins
Week 5 - Practice
Protein precipitation with sulphpsalisilic acid acid test
Week 6 - Theoretical
Derivative proteins
Week 6 - Practice
Protein precipitation with concentrated nitric acid test
Week 7 - Theoretical
Structure and conformation of the protein molecules
Week 7 - Practice
Protein precipitation with triclorasetic asit (TCA) test
Week 8 - Theoretical
Midterm exam
Week 8 - Practice
Midterm evaluation
Week 9 - Theoretical
Denaturation of proteins and precipitation reactions
Week 9 - Practice
Denaturation of proteins and precipitation reactions
Week 10 - Theoretical
Identification with the color reactions of proteins
Week 10 - Practice
Identification of proteins by reaction assay ksantoprotein
Week 11 - Theoretical
Proteins, sorting resolutions
Week 11 - Practice
Identification of proteins by the reaction of lead acetate test
Week 12 - Theoretical
Separating proteins by molecular size
Week 12 - Practice
Identification of proteins by the reaction of lead acetate test
Week 13 - Theoretical
Separation of proteins by electrical charge
Week 13 - Practice
Identification of proteins by reaction assay Sakagucchi
Week 14 - Theoretical
The amino-terminal amino acid of a protein or peptide Assays
Week 14 - Practice
Determination of protein in the blood
Assessment Methods and Criteria
Type of AssessmentCountPercent
Assignment1%5
Quiz1%5
Midterm Examination1%20
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory140114
Lecture - Practice140228
Assignment25112
Term Project22310
Quiz25112
Midterm Examination1819
Final Examination114115
TOTAL WORKLOAD (hours)100
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
PÇ-15
PÇ-16
PÇ-17
PÇ-18
OÇ-1
5
5
5
4
5
OÇ-2
5
5
3
OÇ-3
5
4
4
OÇ-4
5
5
4
4
3
OÇ-5
5
5
4
4
3
Adnan Menderes University - Information Package / Course Catalogue
2026