Information Package / Course Catalogue
PCR and Application Fields
Course Code: VBY644
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 1
Prt.: 2
Credit: 2
Lab: 0
ECTS: 3
Objectives of the Course

The principle of of PCR, some applications of PCR and evaluation of results.

Course Content

The principle of of PCR, some applications of PCR and evaluation of results.

Name of Lecturer(s)
Learning Outcomes
1.Laboratory work to learn the layout.
2.Learn how to use PCR.
3.To learn the application areas of PCR
4.To learn how to prepare samples for PCR applications
5.To learn the advantages and disadvantages of PCR
Recommended or Required Reading
1.PCR Troubleshooting and Optimization: The Essential Guide. Caister Academic Press. Edited by: David Rodriguez-Lazaro
2.A-Z of Quantitative PCR. Stephen A. Bustin.
Weekly Detailed Course Contents
Week 1 - Theoretical
The definition and history of PCR
Week 1 - Practice
Presentation the Central Laboratory
Week 2 - Theoretical
Application areas of PCR
Week 2 - Practice
Presentatiom of equipments
Week 3 - Theoretical
The advantages of PCR
Week 3 - Practice
Preparation of application plan
Week 4 - Theoretical
PCR of the reasons for limiting
Week 4 - Practice
Preparation of tools and equipment
Week 5 - Theoretical
Developments in PCR
Week 5 - Practice
Sample preperation
Week 6 - Theoretical
The basic components of PCR
Week 6 - Practice
Analysis
Week 7 - Theoretical
Types of PCR
Week 7 - Practice
Analysis
Week 8 - Theoretical
Midterm exam
Week 8 - Practice
Analysis
Week 9 - Theoretical
Efficient PCR amplification conditions
Week 9 - Practice
Analysis
Week 10 - Theoretical
The general problems by PCR
Week 10 - Practice
Analysis
Week 11 - Theoretical
Sources of potential contamination of PCR
Week 11 - Practice
Analysis
Week 12 - Theoretical
Needs to be done to prevent contamination of PCR
Week 12 - Practice
Analysis
Week 13 - Theoretical
Potential problems related to pcr
Week 13 - Practice
Evaluation of results
Week 14 - Theoretical
The use of PCR in the diagnosis of diseases
Week 14 - Practice
Discussion
Assessment Methods and Criteria
Type of AssessmentCountPercent
Assignment2%10
Quiz2%10
Midterm Examination1%10
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory140114
Lecture - Practice140228
Assignment2216
Quiz25011
Midterm Examination1718
Final Examination112113
TOTAL WORKLOAD (hours)80
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
PÇ-15
PÇ-16
PÇ-17
PÇ-18
OÇ-1
5
5
4
5
5
3
OÇ-2
5
5
3
OÇ-3
5
5
4
5
3
OÇ-4
5
5
4
5
3
OÇ-5
5
5
4
4
3
Adnan Menderes University - Information Package / Course Catalogue
2026