Information Package / Course Catalogue
Isolation and Purification of Proteins
Course Code: VBY649
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 1
Prt.: 2
Credit: 2
Lab: 0
ECTS: 4
Objectives of the Course

Isolation and purification of proteins used in the methods and their principles. Affinity chromatography, ultrafiltration, electrophoresis, gel filtration, ion exchange chromatography using methods of protein purification.

Course Content

Isolation and purification of proteins used in the methods and their principles. Affinity chromatography, ultrafiltration, electrophoresis, gel filtration, ion exchange chromatography using methods of protein purification.

Name of Lecturer(s)
Learning Outcomes
1.Laboratory work to learn the layout of the
2.Protein determination methods as applied to learn
3.To have information about precipitation of proteins
4.To have knowledge about electrophoresis
5.To have information about chromatography
Recommended or Required Reading
1.Lippincott’s Illustrated Reviews Biyokimya Seri Editörleri Richard A HARVEY, Pamela C. CHAMPE Biyokimya Çeviri Editörü Doç.Dr. Engin ULUKAYA, Nobel Tıp Kitabevleri 2007
2.Lehninger Biyokimyanın İlkeleri. David L. Nelson Michael M. COX. Çeviri Editörü Prof.Dr. Nedret KILIÇ, Palme Yayıncılık
3.Harper Biyokimya Robert K. Murray, Daryl K. Granner, Peter A. Mayes, Victor W. Rodwell. Çeviri Editörleri: Nurten DİKMEN, Tuncay ÖZGÜNEN. Nobel Tıp Kitabevleri
Weekly Detailed Course Contents
Week 1 - Theoretical
İntroduction of protein purification
Week 1 - Practice
Centrifugation
Week 2 - Theoretical
Precipitation of proteins
Week 2 - Practice
Precipitation
Week 3 - Theoretical
Gel filtration
Week 3 - Practice
Dialysis
Week 4 - Theoretical
Ultrafiltration and lyophilization
Week 4 - Practice
Liophilisation
Week 5 - Theoretical
Midterm Exam
Week 5 - Practice
Midterm Exam Evaluation
Week 6 - Theoretical
Protein solutions preconcentration
Week 6 - Practice
Ultrafiltration
Week 7 - Theoretical
Column chromatography
Week 7 - Practice
Column chromatography
Week 8 - Theoretical
Ion exchange chromatography
Week 8 - Practice
Ion exchange chromatography
Week 9 - Theoretical
Affinity chromatography
Week 9 - Practice
Affinity chromatography
Week 10 - Theoretical & Practice
SDS PAGE electrophoresis
Week 11 - Practice
Midterm Exam Evaluation
Week 11 - Intermediate Exam
Midterm Exam
Week 12 - Theoretical
Electrophoresis techniques
Week 12 - Practice
SDS electrophoresis
Week 13 - Theoretical
Isoelectric focusing
Week 13 - Practice
SDS electrophoresis
Week 14 - Theoretical
Blotting techniques
Week 14 - Practice
Western blotting
Assessment Methods and Criteria
Type of AssessmentCountPercent
Assignment4%10
Quiz2%10
Midterm Examination1%10
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory140114
Lecture - Practice140228
Assignment47132
Quiz25011
Midterm Examination1718
Final Examination1617
TOTAL WORKLOAD (hours)100
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
PÇ-15
PÇ-16
PÇ-17
PÇ-18
OÇ-1
5
5
5
5
4
4
4
OÇ-2
5
4
3
OÇ-3
5
5
5
4
4
3
OÇ-4
5
5
5
4
5
3
OÇ-5
5
5
5
4
3
3
Adnan Menderes University - Information Package / Course Catalogue
2026