Information Package / Course Catalogue - Adnan Menderes University

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Enzyme Determination Methods

Course Code	: VBY654
Course Type	: Area Elective
Couse Group	: Third Cycle (Doctorate Degree)
Education Language	: Turkish
Work Placement	: N/A

Theory	: 1
Prt.	: 2
Credit	: 2
Lab	: 0
ECTS	: 3

Objectives of the Course

Purification and identifications of enzymes by chromatographic methods; kinetic calculations

Course Content

Purification and identifications of enzymes by chromatographic methods; kinetic calculations

Name of Lecturer(s)

Learning Outcomes

1.	to learn laboratory processing
2.	to learn enzym assay’s methods
3.	to learn enzym purification
4.	To have knowledge about the biotechnological applications of enzymes
5.	To have information about enzymes and recombinant DNA technology

Recommended or Required Reading

1.	1. Lippincott’s Illustrated Reviews Biyokimya Seri Editörleri Richard A HARVEY, Pamela C. CHAMPE Biyokimya Çeviri Editörü Doç.Dr. Engin ULUKAYA, Nobel Tıp Kitabevleri 2007
2.	Lehninger Biyokimyanın İlkeleri. David L. Nelson Michael M. COX. Çeviri Editörü Prof.Dr. Nedret KILIÇ, Palme Yayıncılık
3.	Harper Biyokimya Robert K. Murray, Daryl K. Granner, Peter A. Mayes, Victor W. Rodwell. Çeviri Editörleri: Nurten DİKMEN, Tuncay ÖZGÜNEN. Nobel Tıp Kitabevleri

Weekly Detailed Course Contents

Week 1 - Theoretical

Structures and functions of enzymes

Week 1 - Practice

Selection of material in the investigate properties of the enzyme

Week 2 - Theoretical

Selection of material in the investigate properties of the enzyme

Week 2 - Practice

Enzyme determination

Week 3 - Theoretical

Extraction and purification enzymes

Week 3 - Practice

Enzyme purification

Week 4 - Theoretical

Extraction and purification enzymes

Week 4 - Practice

Enzim saflaştırılması

Week 5 - Theoretical

Determination of enzymes molecule weight

Week 5 - Practice

Enzyme purification

Week 6 - Theoretical

Principles of enzyme analysis

Week 6 - Practice

Enzyme purification

Week 7 - Theoretical

Midterm exam

Week 8 - Theoretical

Instruments using enzyme analysis

Week 8 - Practice

End-point enzyme anaysis

Week 9 - Theoretical

Automation of enzyme analysis

Week 9 - Practice

Kinetic assays

Week 10 - Theoretical

Enzymes while analytic reactive

Week 10 - Practice

Immunoassay methods

Week 11 - Theoretical

Enzymes analysis in medicine and endustry

Week 11 - Practice

Enzyme histochemistry

Week 12 - Theoretical

Biotecnological applications of enzymes

Week 12 - Practice

Investigation of enzymes in subsellular structures

Week 13 - Theoretical

Biotecnological applications of enzymes

Week 13 - Practice

Storage of enzymes and koenzymes

Week 14 - Theoretical

Enzyme kinetic

Assessment Methods and Criteria

Type of Assessment	Count	Percent
Assignment	2	%5
Quiz	2	%5
Midterm Examination	1	%20
Final Examination	1	%70

Workload Calculation

Activities	Count	Preparation	Time	Total Work Load (hours)
Lecture - Theory	14	0	1	14
Lecture - Practice	14	0	2	28
Laboratory	10	0	1	10
Quiz	2	2	0	5
Midterm Examination	1	6	1	7
Final Examination	1	8	1	9
TOTAL WORKLOAD (hours)				73

Contribution of Learning Outcomes to Programme Outcomes

	PÇ-1	PÇ-2	PÇ-3	PÇ-4	PÇ-5	PÇ-6	PÇ-7	PÇ-8	PÇ-9	PÇ-10	PÇ-11	PÇ-12	PÇ-13	PÇ-14	PÇ-15	PÇ-16	PÇ-17	PÇ-18
OÇ-1	3	4	3					3				4			4		4
OÇ-2				3								3						4
OÇ-3				3	3					4
OÇ-4	3	4	3							4		4					4	4
OÇ-5	3	4	3							4		4					4	4

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