Information Package / Course Catalogue
Method Development and Validation in Pharmacological and Toxicological Analysis
Course Code: VFT671
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 1
Prt.: 2
Credit: 2
Lab: 0
ECTS: 6
Objectives of the Course

Pharmacological and toxicological test measures the importance of the purpose and importance of method validation, statistical calculations used in pharmacological and toxicological measurements, the performance parameters of the method, validation experiments, planning, and reporting the results to re-determining the criteria for validation, transfer and use of validated methods, the system suitability tests and determination of the period of application of knowledge and skills to win.

Course Content

Pharmacology and toxicology analysis methods, chromatographic methods, chromatographic methods, method development, method validation of chromatographic methods.

Name of Lecturer(s)
Learning Outcomes
1.Pharmacology and toxicology analysis methods
2.Chromatographic methods
3.Chromatographic methods, method development
4.Chromatographic method validation methods on the knowledge and skills.
5.To learn knowledge and propose suggestions on the area
Recommended or Required Reading
1.Veterinary Pharmacology and Therapeutics, 8th Edition, Jim E. Riviere (Editor), Mark G. Papich (Editor), 2009.
2.Modern Pharmacology, 6th Edition, Lippincott Williams and Wilkins, 2004 (Ed. C.R. Craig and R.E. Stitzel)
3.Basic and Clinical Pharmacology, 9th Edition, McGraw-Hill, New York, 2004 (Ed. B. Katzung)
4.Goodman and Gilman’s The Pharmacological Basis of Therapeutics 11th Edition, McGraw-Hill, 2006 (Eds. Brunton, Lazo, Parker, Buxton and Blumenthal)
5.Lippincott's Illustrated Reviews: Pharmacology, 3rd Edition, Lippincott Williams and Wilkins, 2005 (Eds. Howard, Mycek, Harvey & Champe)
6.The Veterinary Formulary edited by Yolande Bishop. London Pharmaceutical Press in association with the British Veterinary Association 2001
7.Pharmacology. Franklin A. Ahrens. Baltimore, Md. London Williams & Wilkins 1996.
8.The physiological basis of veterinary clinical pharmacology J. Desmond Baggot. Oxford Blackwell Science 2001.
9.Veterinary pharmaclgy and therapeutics edited by H. Richard Adams. Ames, Iwa Iwa State University Press 2001.
10.Veterinary Drug Therapy, 1994. Ths. B. Barragry.
Weekly Detailed Course Contents
Week 1 - Theoretical
Pharmacology and toxicology analysis methods
Week 1 - Practice
The materials used in pharmacology and toxicology analysis
Week 2 - Theoretical
Chromatographic methods
Week 2 - Practice
ITK application
Week 3 - Theoretical
GC and HPLC
Week 3 - Practice
Parts of the introduction of GK
Week 4 - Theoretical
Liquid-phase extraction methods
Week 4 - Practice
Introduction to HPLC components
Week 5 - Theoretical
Solid phase extraction methods
Week 5 - Practice
HPLC-conditioning
Week 6 - Theoretical
Linearity calculation and preparation of reference standards
Week 6 - Practice
HPLC application
Week 7 - Theoretical
Calculation of the ratio of buy-back
Week 7 - Practice
HPLC application
Week 8 - Theoretical
(Mid-term exam) Discussion
Week 9 - Theoretical
LOD and LOQ calculations
Week 9 - Practice
GK application
Week 10 - Theoretical
Detection range, and precision (precision) calculation
Week 10 - Practice
GK application
Week 11 - Theoretical
Linearity, Specificity / Selectivity
Week 11 - Practice
chromatogram evaluation
Week 12 - Theoretical
To determine the sensitivity and Reality
Week 12 - Practice
chromatogram evaluation
Week 13 - Theoretical
Reproducible results, the calculation producibility
Week 13 - Practice
Liquid-liquid phase extraction
Week 14 - Theoretical
Preparation of SOPs
Week 14 - Practice
The solid-liquid phase extraction
Week 15 - Final Exam
Final
Assessment Methods and Criteria
Type of AssessmentCountPercent
Presentation1%5
Verbal Examination1%5
Midterm Examination1%20
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory143156
Lecture - Practice143277
Midterm Examination1819
Final Examination19110
TOTAL WORKLOAD (hours)152
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
PÇ-15
PÇ-16
OÇ-1
4
5
4
4
5
OÇ-2
3
5
4
4
3
OÇ-3
4
5
4
4
5
OÇ-4
5
5
4
5
5
OÇ-5
5
4
5
4
Adnan Menderes University - Information Package / Course Catalogue
2026