Information Package / Course Catalogue
Histochemical Methods
Course Code: VHE638
Course Type: Required
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 4
Objectives of the Course

The aim of the course is to teach basic histochemical and cytochemical principles, demonstrations of ipids, nucleic acids, proteins, oligosaccharid and polysaccharids, glycolipids and catecholemines

Course Content

Basic histochemical and cytochemical principles. Demonstrations of ipids, nucleic acids, proteins, oligosaccharid and polysaccharids, glycolipids and catecholemines

Name of Lecturer(s)
Learning Outcomes
1.Students learn basic histochemical principles
2.Students learn basic cytochemical principles
3.To apply basic histochemical and cytochemical methods
4.Students learn demonstrations of ipids, nucleic acids, proteins, oligosaccharid and polysaccharids, glycolipids and catecholemines.
5.Students gain demonstration of enzyme
Recommended or Required Reading
Weekly Detailed Course Contents
Week 1 - Theoretical
Basic histochemical and cytochemical principles
Week 1 - Practice
AB (Alcianblue) stain
Week 2 - Theoretical
Basic histochemical and cytochemical principles
Week 2 - Practice
TB (Toluidine blue) stain
Week 3 - Theoretical
Basic histochemical and cytochemical principles
Week 3 - Practice
AF(Aldehit fucsin)/AB stain
Week 4 - Theoretical
Ions
Week 4 - Practice
Silver nitrate reaction
Week 5 - Theoretical
Lipids
Week 5 - Practice
Oil stains (Sudan Black B, Oil Red O)
Week 6 - Theoretical
Nucleic acids
Week 6 - Practice
Acridine Orange, Azur B methods
Week 7 - Theoretical
Proteins
Week 7 - Practice
Tetrazotizebenzidine method
Week 8 - Theoretical & Practice
Repetition of subjects and Midterm exam
Week 9 - Theoretical
Polysaccharids and oligosaccharids
Week 9 - Practice
Periodic Acid Schiff (PAS) reaction
Week 10 - Theoretical
Glycolipids
Week 10 - Practice
Periodic Acid Schiff (PAS) reaction
Week 11 - Theoretical
Catecholemines
Week 11 - Practice
Periodic Acid Schiff (PAS) reaction
Week 12 - Theoretical
Demonstration of the enzymes
Week 12 - Practice
ANAE method
Week 13 - Theoretical
Demonstration of the enzymes
Week 13 - Practice
ANAE method
Week 14 - Theoretical
Article discussion
Week 14 - Practice
Article discussion
Week 15 - Final Exam
Final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Attending Lectures1%5
Assignment1%5
Midterm Examination1%20
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory130226
Lecture - Practice130226
Assignment210122
Midterm Examination110111
Final Examination114115
TOTAL WORKLOAD (hours)100
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
PÇ-15
PÇ-16
PÇ-17
PÇ-18
PÇ-19
PÇ-20
OÇ-1
OÇ-2
5
4
3
OÇ-3
OÇ-4
5
5
3
OÇ-5
5
4
3
Adnan Menderes University - Information Package / Course Catalogue
2026