Information Package / Course Catalogue
Development of Primordial Germ Cells in Mommalion Embrys and Transgenic Technology
Course Code: VHE656
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 0
Credit: 2
Lab: 0
ECTS: 3
Objectives of the Course

Development of primordial germ cells and their migration to the gonadal anlage, production of transgenic animals through pronuclear injection, production of transgenic animals by using embryonic stem cells, applications of the transgenic technology to farm animals.

Course Content

Development of primordial germ cells and their migration to the gonadal anlage, production of transgenic animals through pronuclear injection, production of transgenic animals by using embryonic stem cells, applications of the transgenic technology to farm animals.

Name of Lecturer(s)
Learning Outcomes
1.Gains expert information on cellular and molecular mechanisms govering primordial germ cell development in mammals
2.Learns methods used for the production of chimeric embryos through the transplantation of embryonal germ cells to a recipient embryo.
3.Understands principles of the transgenic technology.
4. Learns methods used for the production of transgenic offspring by using embryonal germ cells.
5.Follow the recent developments in the development of primitive gender cells in mammalian animals and transgenetic technology.
Recommended or Required Reading
Weekly Detailed Course Contents
Week 1 - Theoretical
Mammalian embryo development at pre-implantation stages of development
Week 2 - Theoretical
Origin and differentiation of primordial germ cells
Week 3 - Theoretical
Migration of primordial germ cells to gonads
Week 4 - Theoretical
Growth factors affecting the development and proliferation of primordial germ cells
Week 5 - Theoretical
In vitro culture of primordial germ cells and establishment of embryonal germ cell lines
Week 6 - Theoretical
Charecterization of embryonal germ cell lines
Week 7 - Theoretical
Cryoprotection of embryonal germ cell lines
Week 8 - Theoretical & Practice
Repetition of subjects and Midterm exam
Week 9 - Theoretical
Transfection of eukaryotic cells
Week 10 - Theoretical
Methods used for transferring a foreign gene to embryonal germ cells
Week 11 - Theoretical
Selection of transfected embryonal germ cells
Week 12 - Theoretical
Transfer of genetically modified embryonal germ cells to a recipient embryo
Week 13 - Theoretical
Molecular tests used for the identification of transgenic offspring
Week 14 - Theoretical
Applications of the transgenic technology to farm animals.
Week 15 - Final Exam
Final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Attending Lectures1%5
Assignment1%5
Midterm Examination1%20
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory130113
Reading60212
Midterm Examination115116
Final Examination133134
TOTAL WORKLOAD (hours)75
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
PÇ-15
PÇ-16
PÇ-17
PÇ-18
PÇ-19
PÇ-20
OÇ-1
2
5
4
5
5
3
3
5
4
4
5
4
4
4
3
4
5
5
5
3
OÇ-2
2
5
4
5
5
3
3
5
4
4
5
4
4
4
3
4
5
5
5
3
OÇ-3
2
5
4
5
5
3
3
5
4
4
5
5
4
4
3
4
5
4
5
3
OÇ-4
2
5
4
5
5
3
3
5
4
4
5
4
4
4
3
4
5
5
5
3
OÇ-5
Adnan Menderes University - Information Package / Course Catalogue
2026