Information Package / Course Catalogue
Production of Avian Chimeric Embryos
Course Code: VHE659
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 3
Objectives of the Course

To have the student understand methods for the construction of inter-species avian chimeric embryos using chick and quail embryos and the methods used for the analysis of chimeras

Course Content

Construction of chimeric embryos by transferring either blastodermal cells or primordial germ cells from one embryo to another in the chick and quail, histological, immunohistochemical and molecular methods used for their examination

Name of Lecturer(s)
Learning Outcomes
1.Learns the isolation of blastodermal cells from the chick and quail embryos,
2. Learns the isolation of primordial germ cells from the chick and quail embryos,
3.Learns methods used for the construction of quail-chick chimeras by cell transplantation
4.Learns histological, immunohistochemical and molecular methods used for the examination of resulting chimeras.
5.Applies histological, immunohistochemical and molecular methods used for the examination of resulting chimeras.
Recommended or Required Reading
Weekly Detailed Course Contents
Week 1 - Theoretical
T: Cleavage divisions and development of the avian embryo between ovulation and oviposition
Week 1 - Practice
U: Fertilized and unfertilized egg
Week 2 - Theoretical
T: Establishment of the area pellucida and area opaca regions
Week 2 - Practice
U: Differentiation of the area pellucida and area opaca regions
Week 3 - Theoretical
T: Scoring of the avian embryo at pre-streak stages of development-I (Eyal-Giladi and Kochav staging system)
Week 3 - Practice
U: Preperation of solutions and equipment used for embryo isolation
Week 4 - Theoretical
T: Scoring of the avian embryo at pre-streak stages of development-II (Eyal-Giladi and Kochav staging system)
Week 4 - Practice
U: Isolation of the embryo at the blastoderm stage
Week 5 - Theoretical
T: Scoring of the avian embryo at post-streak stages of development-I (Hamburger and Hamilton staging system)
Week 5 - Practice
U: Isolation of the embryo at the blastoderm stage
Week 6 - Theoretical
T: Scoring of the avian embryo at post-streak stages of development-II (Hamburger and Hamilton staging system)
Week 6 - Practice
U: Isolation of the embryo at the blastoderm stage
Week 7 - Theoretical
T: Methods used for the construction of quail-chick chimeric embryos-1
Week 7 - Practice
U: Removal of the area pellucid from the area opaca by cutting
Week 8 - Theoretical & Practice
Repetition of subjects and Midterm exam
Week 9 - Theoretical
T: Methods used for the construction of quail-chick chimeric embryos-2
Week 9 - Practice
U: Methodology for obtaining a single cell suspension
Week 10 - Theoretical
T: Significance of the quail-chick chimeric embryo model
Week 10 - Practice
U: Determination of cell counts and cell viability in single cell suspensions
Week 11 - Theoretical
T: Possible uses of the the quail-chick chimeric embryo model
Week 11 - Practice
U: Transplantation of quail blastodermal cells into chick embryos
Week 12 - Theoretical
T: Histological methods used in the analysis of chimeric embryos
Week 12 - Practice
U: Identication of quail cells in putative quail-chick chimeras using Feulgen staining
Week 13 - Theoretical
T: Immunohistochemical methods used in the analysis of chimeric embryos
Week 13 - Practice
U: Identication of quail cells in putative quail-chick chimeras using immunohistochemistry
Week 14 - Theoretical
T: Article discussion
Week 14 - Practice
U: Article presentation
Week 15 - Final Exam
Final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Attending Lectures1%5
Assignment1%5
Midterm Examination1%20
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory130226
Lecture - Practice130226
Midterm Examination19110
Final Examination112113
TOTAL WORKLOAD (hours)75
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
PÇ-13
PÇ-14
PÇ-15
PÇ-16
PÇ-17
PÇ-18
PÇ-19
PÇ-20
OÇ-1
2
5
3
1
4
3
3
4
4
2
2
5
4
3
5
3
3
1
4
4
OÇ-2
2
5
3
1
4
3
3
4
4
2
2
5
4
3
5
3
3
1
4
4
OÇ-3
2
5
3
1
4
3
3
4
4
2
2
5
4
3
5
3
3
1
4
4
OÇ-4
2
5
3
1
4
3
3
4
4
2
2
5
4
3
5
3
3
1
4
4
OÇ-5
Adnan Menderes University - Information Package / Course Catalogue
2026