Information Package / Course Catalogue - Adnan Menderes University

General Information Unit Staff Academic Information Programme Outcomes Course Structure Diagram Contact Information

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Pruduction of Mammalian Chimeric Embryos

Course Code	: VHE660
Course Type	: Area Elective
Couse Group	: Third Cycle (Doctorate Degree)
Education Language	: Turkish
Work Placement	: N/A

Theory	: 2
Prt.	: 0
Credit	: 2
Lab	: 0
ECTS	: 4

Objectives of the Course

To have the student understand methods for the construction of inter-species avian chimeric embryos using mouse as a commonly used mammalian model and the methods used for the analysis of chimeras

Course Content

Construction of mouse chimeric embryos by either through the fusion of two embryos at the morula stage or through the transfer of embryonic stem cells into the blastocoelic cavity and histological, immunohistochemical and molecular methods used for their examination

Name of Lecturer(s)

Learning Outcomes

1.	Learns to construct chimeric embryos through the fusion of two embryos at the morula stage.
2.	Learns principles of techniques for the construction of mouse chimeric embryos through the transfer of embryonic stem cells into the blastocoelic cavity
3.	Learns histological methods used for the examination of resulting chimeras. Düzelt Sil
4.	Learns immunohistochemical methods used for the examination of resulting chimeras.
5.	Learns molecular methods used for the examination of resulting chimeras.

Recommended or Required Reading

Weekly Detailed Course Contents

Week 1 - Theoretical

T: Mammalian embryo development at pre-implantation stages-I

Week 2 - Theoretical

T: Mammalian embryo development at pre-implantation stages-2

Week 3 - Theoretical

T: Mammalian embryo development at pre-implantation stages-3

Week 4 - Theoretical

T: Sexual cycle in mice

Week 5 - Theoretical

T: Superovulation in mice

Week 6 - Theoretical

T: Definition of chimeric embryos and their possible uses in experimental embryology

Week 7 - Theoretical

T: Laboratory methods used for the construction of chicmeric embryos

Week 8 - Theoretical & Practice

Repetition of subjects and Midterm exam

Week 9 - Theoretical

T: Construction of aggregation chimeras in mice

Week 10 - Theoretical

T: Cell types used for the construction of aggregation chimeras in mice

Week 11 - Theoretical

T: Construction of chimeric mouse embryos through the transfer of cells into the blastocoelic cavity

Week 12 - Theoretical

T: Development of transgenic mice through the construction of chimeric embryos-1

Week 13 - Theoretical

T: Application of histological and immunohistochemical methods used for the examination of chimeric embryos

Week 14 - Theoretical

T: Application of molecular methods used for the examination of chimeric embryos

Week 15 - Final Exam

Final exam

Assessment Methods and Criteria

Type of Assessment	Count	Percent
Attending Lectures	1	%5
Assignment	1	%5
Midterm Examination	1	%20
Final Examination	1	%70

Workload Calculation

Activities	Count	Preparation	Time	Total Work Load (hours)
Lecture - Theory	13	0	2	26
Seminar	1	0	8	8
Project	1	0	12	12
Midterm Examination	1	20	1	21
Final Examination	1	32	1	33
TOTAL WORKLOAD (hours)				100

Contribution of Learning Outcomes to Programme Outcomes

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PÇ-5

PÇ-6

PÇ-7

PÇ-8

PÇ-9

PÇ-10

PÇ-11

PÇ-12

PÇ-13

PÇ-14

PÇ-15

PÇ-16

PÇ-17

PÇ-18

PÇ-19

PÇ-20

OÇ-1

OÇ-2

OÇ-3

OÇ-4

OÇ-5

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