Information Package / Course Catalogue
Production Methods Of Haemoparasites In Cell Culture
Course Code: VPR658
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 2
Prt.: 2
Credit: 3
Lab: 0
ECTS: 4
Objectives of the Course

The objective of this course, introduction of cell culture laboratories, points to be considered in cell culture, cell line in cell culture, cultivation of theileria and babesia parasites

Course Content

Cell culture of theileria and babesia parasites, Isolation of infected lymphocytes from the blood, schizonts-infected lymphocyte cultures, cell line and incubation, to be considered in cell culture, cell counting and viability examination, storage of cultures

Name of Lecturer(s)
Learning Outcomes
1.Equipment / consumables used in laboratories cell culture
2.Isolation of peripheral blood monocytes
3.To have an information about cultivation of Theileria parasites
4.To have an information about cultivation of Babesia parasites
5.Cell line and storage of cultures in cell culture
Recommended or Required Reading
1.Maramorosch K., Hirumi, H. (1979). Practical tissue culture applications. Academic Press, USA
Weekly Detailed Course Contents
Week 1 - Theoretical
Points to be considered in cell culture
Week 1 - Practice
equipment / consumables used in laboratories cell culture
Week 2 - Theoretical
Biology of theileria annulata
Week 2 - Practice
Isolation to be lymphocytes of an infected animal
Week 3 - Theoretical
Isolation to be lymphocytes of an infected animal with theileria annulata
Week 3 - Practice
culturing lymphocyte -infected wtith Scizont by Obtained from an animal infected with theileria annulata
Week 4 - Theoretical
Culturing lymphocytes infected with Schizonts
Week 4 - Practice
Culturing lymphocyte -infected wtith Scizont by Obtained from an animal infected with theileria annulata
Week 5 - Theoretical
Cell line and incubation in cell culture of theileria annulata
Week 5 - Practice
Cell line and incubation in cell culture of theileria annulata
Week 6 - Theoretical
Count of cells in cell cultures of theileria annulata and live / dead cell discrimination
Week 6 - Practice
Count of cells in cell cultures of theileria annulata and live / dead cell discrimination
Week 7 - Theoretical
Storage of cell cultures of theileria annulata
Week 7 - Practice
Storage of cell cultures of theileria annulata
Week 8 - Theoretical & Practice
Discussion (Midterm Examination)
Week 9 - Theoretical
Biology of the species Babesia parasites which were cell culturable
Week 9 - Practice
Modified of cell cultures to babesia
Week 10 - Theoretical
Needs to be done for cell culture to babesia
Week 10 - Practice
Modified of cell cultures to babesia
Week 11 - Theoretical
Cell line of cell culture to babesia
Week 11 - Practice
Cell line of cell culture to babesia
Week 12 - Theoretical
Incubation of cell culture to babesia
Week 12 - Practice
Incubation of cell culture to babesia
Week 13 - Theoretical
Storage of cell cultures to babesia
Week 13 - Practice
Storage of cell cultures to babesia
Week 14 - Theoretical
Immunization activities from cell cultures
Week 14 - Practice
Immunization activities from cell cultures
Week 15 - Final Exam
Final exam
Week 16 - Final Exam
Final exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Assignment1%5
Quiz1%5
Midterm Examination1%20
Final Examination1%70
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory140228
Lecture - Practice140228
Assignment1415
Quiz1415
Midterm Examination114115
Final Examination118119
TOTAL WORKLOAD (hours)100
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
PÇ-12
OÇ-1
5
4
5
4
5
4
5
4
4
5
OÇ-2
5
4
5
4
5
4
5
4
4
5
OÇ-3
5
4
5
4
5
4
5
4
4
5
OÇ-4
5
4
5
4
5
4
5
4
4
5
OÇ-5
5
4
5
4
5
4
5
4
4
5
Adnan Menderes University - Information Package / Course Catalogue
2026