Information Package / Course Catalogue
Special Laboratory Methods
Course Code: TPR645
Course Type: Area Elective
Couse Group: Third Cycle (Doctorate Degree)
Education Language: Turkish
Work Placement: N/A
Theory: 1
Prt.: 2
Credit: 2
Lab: 0
ECTS: 2
Objectives of the Course

The special tecniques that are not used in routine examination for staning like Acridine orange or like modify tricrom for microsporidium, for culture such as Karapetyan, HSP-1, HSP-2  media and for diagnostic tests like buffy coat concentration  are tought and making practically

Course Content

The special tecniques that are not used in routine examination for staning like Acridine orange or like modify tricrom for microsporidium, for culture such as Karapetyan, HSP-1, HSP-2  media and for diagnostic tests like buffy coat concentration  are tought and making practically

Name of Lecturer(s)
Learning Outcomes
1. Acridine orange staining method, modified to apply the methods of staining trichrome
2.Karapetyan, HSP-1, HSP-2 medium to make
3.buffy coat concentration, microhematocrit centrifuge, to apply the methods of Knott
4.Application of the technique of protein analysis and SDS-PAGE
5. Application of the technique of Western Blot
6.Application of the technique of PCR
7.Application of the technique of RFLP
Recommended or Required Reading
1.Lawrence RA, Orihel TC (eds) 1987. Parasites: a guide to laboratory procedures and identification.ASCP press. Chicago. ISBN 0-89189-231-1
2.Ozcel MA, N Altıntaş (eds) 1997. Parazit Hastalıklarında Tanı: Türkiye Parazitoloji Derneği Yayın No:15.Izmir
Weekly Detailed Course Contents
Week 1 - Theoretical & Practice
Introduction to special techniques
Week 2 - Theoretical & Practice
Acridine orange staining method, Modified trichrome
Week 3 - Theoretical & Practice
Karapetyan, HSP-1, HSP-2 medium
Week 4 - Theoretical & Practice
buffy coat concentration, microhematocrit centrifuge, to apply the methods of Knott
Week 5 - Theoretical & Practice
Protein analysis and SDS-PAGE
Week 6 - Theoretical & Practice
Western blot method
Week 7 - Theoretical & Practice
The complement fixation method
Week 8 - Theoretical & Practice
Molecular Biological Methods (Midterm exam)
Week 9 - Theoretical & Practice
Molecular Biological Methods
Week 10 - Theoretical & Practice
Classical molecular methods
Week 11 - Theoretical & Practice
Amplification techniques
Week 12 - Theoretical & Practice
Real Time PCR
Week 13 - Theoretical & Practice
Gene cloning techniques
Week 14 - Theoretical
Gene cloning techniques II
Week 15 - Final Exam
Final Exam
Assessment Methods and Criteria
Type of AssessmentCountPercent
Assignment1%5
Quiz1%5
Midterm Examination1%30
Final Examination1%60
Workload Calculation
ActivitiesCountPreparationTimeTotal Work Load (hours)
Lecture - Theory4014
Lecture - Practice150115
Seminar8018
Project1033
Laboratory8018
Studio Work5015
Quiz4014
Midterm Examination1011
Final Examination1022
TOTAL WORKLOAD (hours)50
Contribution of Learning Outcomes to Programme Outcomes
PÇ-1
PÇ-2
PÇ-3
PÇ-4
PÇ-5
PÇ-6
PÇ-7
PÇ-8
PÇ-9
PÇ-10
PÇ-11
OÇ-1
3
3
3
3
3
3
3
3
3
3
3
OÇ-2
OÇ-3
OÇ-4
OÇ-5
OÇ-6
OÇ-7
Adnan Menderes University - Information Package / Course Catalogue
2026