To have knowledge about cell culture properties, preparation of materials, special techniques, and cell culture applications.

Primary cultures, short-and long-term cultures. Cultures care, primary cultures, cell line, defining the concepts of continuous cell lines, changing of the media and cell passages, different purposes culture conditions. Characterization of cells in culture, markers of tissue and concepts of transformation. Staining, microscopy and photography for the morphological evalution purposes. Contamination and types of microbial contamination, contamination detection methods, methods for the determination of mycoplasma contamination, kros contamination and prevention methods.